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Fluorescence and super-resolution standards based on DNA origami

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Figure 1: DNA origami standards for fluorescence microscopy.


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We thank V. Schüller, R. Schreiber, E. Pibiri, I. Burkhardt, R. Jungmann, P. Nickels, T. Liedl, B. Lalkens and D. Grohmann for help with experiments and fruitful discussions. We are grateful to W. Fouquet, J. Sieber and Leica Microsystems for STED measurements. Financial support by the Biophotonics IV program of the Federal Ministry of Education and Research (BMBF)/Association of German Engineers (VDI)(13N11461) and the German Research Foundation (DFG Ti329/6-1) is gratefully acknowledged.

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Correspondence to Philip Tinnefeld.

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Braunschweig University of Technology, which J.J.S., A.G., P.H., C.F., T.D. and P.T. are affiliated with, has filed a provisional patent application for DNA origami–based standards.

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Schmied, J., Gietl, A., Holzmeister, P. et al. Fluorescence and super-resolution standards based on DNA origami. Nat Methods 9, 1133–1134 (2012).

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