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Gene expression

Timing an intron's departure

Nature Methods volume 9, pages 122–123 (2012)Cite this article

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An in vitro RNA-labeling technique with single-molecule resolution offers a look into the kinetics and the location of splicing.

“People believe, and it is generally true,” explains Tyagi, “that most splicing takes place co-transcriptionally..., but much of splicing is alternative splicing, and this cannot happen if you remove the intron as soon as it is made.”

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References

  1. Raj, A. et al. Imaging individual mRNA molecules using multiple singly labeled probes. Nat. Methods 5, 877–879 (2008).

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  2. Vargas, D.Y. et al. Single-molecule imaging of transcriptionally coupled ad uncoupled splicing. Cell 147, 1054–1065 (2011).

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  1. Nicole Rusk
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Rusk, N. Timing an intron's departure. Nat Methods 9, 122–123 (2012). https://doi.org/10.1038/nmeth.1882

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