Optimal enzymes for amplifying sequencing libraries

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Figure 1: Genome coverage uniformity plots for 10× Illumina sequence coverage.

References

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Correspondence to Michael A Quail.

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The authors declare no competing financial interests.

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Supplementary Text and Figures

Supplementary Figures 1–3, Supplementary Table 1, Supplementary Methods (PDF 2134 kb)

Supplementary Table 2

Enzymes and conditions used for amplification step in Illumina library construction. (XLSX 20 kb)

Supplementary Table 3

Four-genome alternative enzyme study. Rank order. (XLSX 26 kb)

Supplementary Table 4

Four-genome alternative enzyme study. Performance ranking based on coverage and fidelity. (XLSX 104 kb)

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Quail, M., Otto, T., Gu, Y. et al. Optimal enzymes for amplifying sequencing libraries. Nat Methods 9, 10–11 (2012). https://doi.org/10.1038/nmeth.1814

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