Optimal enzymes for amplifying sequencing libraries

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Genome coverage uniformity plots for 10× Illumina sequence coverage.


  1. 1

    Kozarewa, I. et al. Nat. Methods 6, 291–295 (2009).

    CAS  Article  Google Scholar 

  2. 2

    Quail, M. et al. Nat. Methods 5, 1005–1010 (2008).

    CAS  Article  Google Scholar 

  3. 3

    Aird, D. et al. Genome Biol. 12, R18 (2011).

    CAS  Article  Google Scholar 

  4. 4

    Fisher, S. et al. Genome Biol. 12, R1 (2011).

    Article  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Michael A Quail.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–3, Supplementary Table 1, Supplementary Methods (PDF 2134 kb)

Supplementary Table 2

Enzymes and conditions used for amplification step in Illumina library construction. (XLSX 20 kb)

Supplementary Table 3

Four-genome alternative enzyme study. Rank order. (XLSX 26 kb)

Supplementary Table 4

Four-genome alternative enzyme study. Performance ranking based on coverage and fidelity. (XLSX 104 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Quail, M., Otto, T., Gu, Y. et al. Optimal enzymes for amplifying sequencing libraries. Nat Methods 9, 10–11 (2012). https://doi.org/10.1038/nmeth.1814

Download citation

Further reading


Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing