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Optimal enzymes for amplifying sequencing libraries

Nature Methods volume 9, pages 10–11 (2012)Cite this article

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Figure 1: Genome coverage uniformity plots for 10× Illumina sequence coverage.

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Authors and Affiliations

  1. Wellcome Trust Sanger Institute, Hinxton, UK

    Michael A Quail, Thomas D Otto, Yong Gu, Simon R Harris, Thomas F Skelly, Jacqueline A McQuillan, Harold P Swerdlow & Samuel O Oyola

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  1. Michael A Quail
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  2. Thomas D Otto
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  3. Yong Gu
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  4. Simon R Harris
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  7. Harold P Swerdlow
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  8. Samuel O Oyola
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Correspondence to Michael A Quail.

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Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–3, Supplementary Table 1, Supplementary Methods (PDF 2134 kb)

Supplementary Table 2

Enzymes and conditions used for amplification step in Illumina library construction. (XLSX 20 kb)

Supplementary Table 3

Four-genome alternative enzyme study. Rank order. (XLSX 26 kb)

Supplementary Table 4

Four-genome alternative enzyme study. Performance ranking based on coverage and fidelity. (XLSX 104 kb)

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Quail, M., Otto, T., Gu, Y. et al. Optimal enzymes for amplifying sequencing libraries. Nat Methods 9, 10–11 (2012). https://doi.org/10.1038/nmeth.1814

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