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Zinc-finger nucleases: how to play two good hands

Zinc-finger nuclease dimers are more difficult to engineer than single DNA-binding domains, but the development of new methods could help.

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Figure 1: Overcoming the challenges for engineering functional zinc-finger nucleases.
Figure 2: Screening for full zinc-finger nuclease activity.

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Acknowledgements

M.I. is funded by the European Research Council, FP7-ERC-201249-ZINC-HUBS, Ministerio de Ciencia e Innovacion grant MICINN BFU2010-17953, and The Ministerio de Educacion y Ciencia and European Molecular Biology Laboratory (MEC-EMBL) agreement.

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Isalan, M. Zinc-finger nucleases: how to play two good hands. Nat Methods 9, 32–34 (2012). https://doi.org/10.1038/nmeth.1805

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