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Toward the blood-borne miRNome of human diseases


In a multicenter study, we determined the expression profiles of 863 microRNAs by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and validated this 'miRNome' by quantitative real-time PCR. We detected consistently deregulated profiles for all tested diseases; pathway analysis confirmed disease association of the respective microRNAs. We observed significant correlations (P = 0.004) between the genomic location of disease-associated genetic variants and deregulated microRNAs.

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Figure 1: Bubble plot of miRNAs that are up- or downregulated in several diseases.
Figure 2: Representative example for the physical proximity of a significantly deregulated miRNA and a known SNP.

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Gene Expression Omnibus


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We thank F. Flachsbart, B. Noack and B. Loos for support. This work was financially supported by the German Ministry of Research Education (Bundesministerium für Bildung und Forschung 01EX0806), Hedwig Stalter Foundation, Homburger Forschungsförderungsprogramm and by the Deutsche Forschungsgemeinschaft (LE2783/1-1). Infrastructure support was received from the Deutsche Forschungsgemeinschaft cluster of excellence 'Inflammation at Interfaces'.

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Authors and Affiliations



A.K. initiated the study; E.M., P.R., J.M-Q., A.B., P.S., V.B., C.S., M.B., M.W.B., J.W., S.F.M.H., J.D., S.S., H.A.K., W.R., B.M., J.D.H. and A.F. designed the study; A.K., P.L., A.E., H.A.K., W.R., B.M., J.D.H., A.F., E.M., S.S. and B.V. wrote the manuscript; A.K., J.H., C.B., A.W., I.A., B.V. and H-P.L. analyzed data; P.L., A.B., C.T., A.E., N.G., K.O., J.W., T.H., G.J., H.D., A.S., B.W., B.K., N.G., A.N., V.B., B.V., S.H. and B.M. performed experiments; C.T., K.O., T.H., K.R., H.H., J.H., G.J., H.D., A.S., B.W., B.K., J.R., S.U.J., N.G., M.S., M.W.B., J.W. and S.F.M.H. collected samples.

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Correspondence to Andreas Keller.

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Competing interests

A.K., C.B., A.W., I.A., P.S., V.B., C.S., M.B., MS have been affiliated with febit, a biotech company specializing in microarray screening.

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Supplementary Figures 1–6, Supplementary Tables 1–8 (PDF 5051 kb)

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Keller, A., Leidinger, P., Bauer, A. et al. Toward the blood-borne miRNome of human diseases. Nat Methods 8, 841–843 (2011).

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