The two-color traffic light reporter reads out what pathway is used to repair a DNA break and will increase insights into genome engineering.
This is a preview of subscription content, access via your institution
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Rent or buy this article
Prices vary by article type
Prices may be subject to local taxes which are calculated during checkout
Certo, M.T. et al. Nat. Methods 8, 671–676 (2011).
Wigler, M. et al. Cell 16, 777–785 (1979).
Doetschman, T. et al. Nature 330, 576–578 (1987).
Thomas, K.R. & Capecchi, M.R. Cell 51, 503–512 (1987).
Jasin, M. Trends Genet. 12, 224–228 (1996).
Kim, Y.G., Cha, J. & Chandrasegaran, S. Proc. Natl. Acad. Sci. USA 93, 1156–1160 (1996).
Porteus, M.H. & Baltimore, D. Science 300, 763 (2003).
Urnov, F.D., Rebar, E.J., Holmes, M.C., Zhang, H.S. & Gregory, P.D. Nat. Rev. Genet. 11, 636–646 (2010).
Wyman, C. & Kanaar, R. Annu. Rev. Genet. 40, 363–383 (2006).
Pruett-Miller, S.M., Connelly, J.P., Maeder, M.L., Joung, J.K. & Porteus, M.H. Mol. Ther. 16, 707–717 (2008).
Potts, P.R., Porteus, M.H. & Yu, H. EMBO J. 25, 3377–3388 (2006).
The author declares no competing financial interests.
About this article
Cite this article
Porteus, M. Seeing the light: integrating genome engineering with double-strand break repair. Nat Methods 8, 628–630 (2011). https://doi.org/10.1038/nmeth.1656