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Generating high-quality protein binders: a large screening effort pays off

Nature Methods volume 8, pages 545–546 (2011)Cite this article

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A large-scale, multifaceted screening and validation strategy combining traditional and recombinant antibody technologies yielded a broad spectrum of validated protein binders with high binding affinity and specificity applicable to many fields in proteome research.

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Figure 1: Pipeline for the generation of high-quality protein binders to SH2 domains.

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Authors and Affiliations

  1. Peter Nollau is at University Medical Center Hamburg–Eppendorf, Institute of Clinical Chemistry, Hamburg, Germany.,

    Peter Nollau

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  1. Peter Nollau
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Correspondence to Peter Nollau.

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The author declares no competing financial interests.

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Nollau, P. Generating high-quality protein binders: a large screening effort pays off. Nat Methods 8, 545–546 (2011). https://doi.org/10.1038/nmeth.1632

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