A transposon-insertion method can trap and mutate genes while simultaneously revealing their spatiotemporal expression pattern, all in a conditional manner in the live, transparent zebrafish.
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References
Clark, K.J. et al. Nat. Methods 8, 506–512 (2011).
Friedel, R.H. & Soriano, P. Methods Enzymol. 477, 243–269 (2010).
Schnutgen, F. et al. Proc. Natl. Acad. Sci. USA 102, 7221–7226 (2005).
Boniface, E.J. et al. Genesis 47, 484–491 (2009).
Guan, C. et al. Genesis 48, 73–85 (2010).
Ingham, P.W. Hum. Mol. Genet. 6, 1755–1760 (1997).
Amsterdam, A. & Hopkins, N. Trends Genet. 22, 473–480 (2006).
Moens, C.B. et al. Brief. Funct. Genomics Proteomics 7, 454–459 (2008).
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Mullins, M. All-in-one live: genes trapped, tagged and conditionally broken. Nat Methods 8, 466–467 (2011). https://doi.org/10.1038/nmeth.1617
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DOI: https://doi.org/10.1038/nmeth.1617