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Chemical synthesis of the mouse mitochondrial genome


We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

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Figure 1: Schematic demonstrating assembly of the synthetic mouse mitochondrial genome.
Figure 2: Summary of results for obtaining the 75 sequence-verified first-stage assemblies.

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  1. Agarwal, K.L. et al. Nature 227, 27–34 (1970).

    Article  CAS  Google Scholar 

  2. Gibson, D.G. et al. Science 319, 1215–1220 (2008).

    Article  CAS  Google Scholar 

  3. Gibson, D.G. et al. Science 329, 52–56 (2010).

    Article  CAS  Google Scholar 

  4. Kodumal, S.J. et al. Proc. Natl. Acad. Sci. USA 101, 15573–15578 (2004).

    Article  CAS  Google Scholar 

  5. Welch, M. et al. PLoS ONE 4, e7002 (2009).

    Article  Google Scholar 

  6. Xiong, A.S. et al. FEMS Microbiol. Rev. 32, 522–540 (2008).

    Article  CAS  Google Scholar 

  7. Gibson, D.G. et al. Nat. Methods 6, 343–345 (2009).

    Article  CAS  Google Scholar 

  8. Tian, J. et al. Nature 432, 1050–1054 (2004).

    Article  CAS  Google Scholar 

  9. Meldrum, D. Genome Res. 10, 1081–1092 (2000).

    Article  CAS  Google Scholar 

  10. Itaya, M. et al. Nat. Methods 5, 41–43 (2008).

    Article  CAS  Google Scholar 

  11. Taylor, R.W. & Turnbull, D.M. Nat. Rev. Genet. 6, 389–402 (2005).

    Article  CAS  Google Scholar 

  12. Cox, J.C. et al. Protein Sci. 16, 379–390 (2007).

    Article  CAS  Google Scholar 

  13. Lee, C.C., Snyder, T.M. & Quake, S.R. Nucleic Acids Res. 38, 2514–2521 (2010).

    Article  CAS  Google Scholar 

  14. Khalil, A.S. & Collins, J.J. Nat. Rev. Genet. 11, 367–379 (2010).

    Article  CAS  Google Scholar 

  15. Thompson, J.D., Higgins, D.G. & Gibson, T.J. Nucleic Acids Res. 22, 4673–4680 (1994).

    Article  CAS  Google Scholar 

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We thank Synthetic Genomics, Inc. for funding this work, J. Glass, Y.-H. Rogers, J. Gill, M. Frazier, E. Eisenstadt and M. Gibson for helpful discussions, and M. Algire and J. Zaveri for technical assistance.

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Authors and Affiliations



D.G.G. and C.M. designed research, performed research, analyzed data and wrote the paper. H.O.S., C.A.H. III and J.C.V. designed research and analyzed data.

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Correspondence to Daniel G Gibson or Chuck Merryman.

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Competing interests

J.C.V. is chief executive officer and co-chief scientific officer of Synthetic Genomics, Inc (SGI). H.O.S. is co-chief scientific officer and a member of the board of directors of SGI. C.A.H. III is chairman of the SGI Scientific Advisory Board. J.C.V., H.O.S. and C.A.H. III hold SGI stock.

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Supplementary Figures 1–6, Supplementary Tables 1–15, Supplementary Note 1 (PDF 1416 kb)

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Gibson, D., Smith, H., Hutchison, C. et al. Chemical synthesis of the mouse mitochondrial genome. Nat Methods 7, 901–903 (2010).

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