Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red-shifted emission maximum and high photostability.
This is a preview of subscription content, access via your institution
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Rent or buy this article
Prices vary by article type
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Hoffman, R.M. J. Biomed. Opt. 10, 41202 (2005).
Shcherbo, D. et al. Nat. Methods 4, 741–746 (2007).
Deliolanis, N.C. et al. J. Biomed. Opt. 13, 044008 (2008).
Shu, X. et al. Science 324, 804–807 (2009).
Strack, R.L. et al. Nat. Methods 5, 955–957 (2008).
Lin, M.Z. et al. Chem. Biol. 16, 1169–1179 (2009).
Pletnev, S. et al. J. Biol. Chem. 283, 28980–28987 (2008).
Strack, R.L. et al. Biochemistry 48, 8279–8281 (2009).
Shaner, N.C. et al. Nat. Biotechnol. 22, 1567–1572 (2004).
Kredel, S. et al. Chem. Biol. 15, 224–233 (2008).
Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K. & Pease, L.R. Gene 77, 51–59 (1989).
Shcherbo, D. et al. Biochem. J. 418, 567–574 (2009).
Gross, L.A., Baird, G.S., Hoffman, R.C., Baldridge, K.K. & Tsien, R.Y. Proc. Natl. Acad. Sci. USA 97, 11990–11995 (2000).
Smith, M.C. et al. Cancer Res. 64, 8604–8612 (2004).
Luker, G.D. et al. J. Virol. 76, 12149–12161 (2002).
We thank J.M. Steele for technical assistance with mouse experiments and B.S. Glick (University of Chicago) for providing the plasmid encoding E2-Crimson. This work was supported by grants from the Molecular and Cell Biology program of the Russian Academy of Sciences, the Howard Hughes Medical Institute (55005618), the US National Institutes of Health (R01CA136553, R01CA136829 and P50CA093990), Rosnauka (02.512.12.2053) and the Russian Foundation for Basic Research (08-04-01702-à and 10-04-01042).
eqFP650 and eqFP670 are property of Evrogen JSC, Moscow, Russia. K.A.L. and D.M.C. have interest in Evrogen JSC. T.V.G. is employed by Evrogen JSC.
About this article
Cite this article
Shcherbo, D., Shemiakina, I., Ryabova, A. et al. Near-infrared fluorescent proteins. Nat Methods 7, 827–829 (2010). https://doi.org/10.1038/nmeth.1501
This article is cited by
In vivo long-term investigation of tumor bearing mKate2 by an in-house fluorescence molecular imaging system
BioMedical Engineering OnLine (2018)
Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion
Biophysics Reports (2018)
Synergistic Strategy for Multicolor Two-photon Microscopy: Application to the Analysis of Germinal Center Reactions In Vivo
Scientific Reports (2017)
Current Cardiovascular Imaging Reports (2017)
Bulletin of Experimental Biology and Medicine (2017)