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Near-infrared fluorescent proteins

Nature Methods volume 7, pages 827–829 (2010)Cite this article

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Abstract

Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red-shifted emission maximum and high photostability.

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Figure 1: Cytotoxicity and spectral characteristics.
Figure 2: Whole-mouse imaging with IVIS Spectrum system (Caliper).
Figure 3: Whole-mouse infrared imaging with the Biospec system.

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GenBank/EMBL/DDBJ

Protein Data Bank

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Acknowledgements

We thank J.M. Steele for technical assistance with mouse experiments and B.S. Glick (University of Chicago) for providing the plasmid encoding E2-Crimson. This work was supported by grants from the Molecular and Cell Biology program of the Russian Academy of Sciences, the Howard Hughes Medical Institute (55005618), the US National Institutes of Health (R01CA136553, R01CA136829 and P50CA093990), Rosnauka (02.512.12.2053) and the Russian Foundation for Basic Research (08-04-01702-à and 10-04-01042).

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Author notes

  1. Dmitry Shcherbo and Irina I Shemiakina: These authors contributed equally to this work.

Authors and Affiliations

  1. Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia

    Dmitry Shcherbo, Irina I Shemiakina, Ekaterina A Souslova, Lydia Strukova, Konstantin M Shidlovskiy, Olga V Britanova, Andrey G Zaraisky, Konstantin A Lukyanov & Dmitriy M Chudakov

  2. A.M. Prokhorov General Physics Institute, Russian Academy of Science, Moscow, Russia

    Anastasiya V Ryabova & Victor B Loschenov

  3. Center for Molecular Imaging, University of Michigan Medical School, Ann Arbor, Michigan, USA

    Kathryn E Luker, Bradley T Schmidt & Gary D Luker

  4. Evrogen JSC, Moscow, Russia

    Tatiana V Gorodnicheva

  5. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA

    Gary D Luker

Authors
  1. Dmitry Shcherbo
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  15. Dmitriy M Chudakov
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Contributions

D.S., I.I.S. and L.S. developed fluorescent proteins. A.V.R. and V.B.L. performed experiments on a Biospec imaging system. K.E.L., B.T.S. and G.D.L. performed experiments on a Caliper IVIS spectrum imaging system. A.G.Z. performed experiments on Xenopus embryos. T.V.G. and E.A.S. grew cells and performed microscopy experiments. K.M.S. generated transgenic zebrafish. O.V.B. performed flow cytometry analysis. K.A.L., G.D.L. and D.M.C. designed and planned the project and wrote the manuscript.

Corresponding author

Correspondence to Dmitriy M Chudakov.

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Competing interests

eqFP650 and eqFP670 are property of Evrogen JSC, Moscow, Russia. K.A.L. and D.M.C. have interest in Evrogen JSC. T.V.G. is employed by Evrogen JSC.

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Shcherbo, D., Shemiakina, I., Ryabova, A. et al. Near-infrared fluorescent proteins. Nat Methods 7, 827–829 (2010). https://doi.org/10.1038/nmeth.1501

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