Direct observation of haplotypes is still technically challenging. Here we report a method for the determination of haplotypes through chromosome microdissection. We determined human long-range chromosomal haplotypes with more than 98.85% accuracy at 24,245 genome-wide heterozygous single-nucleotide polymorphism (SNP) loci.
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This work was supported by US National Institutes of Health grants (HL003676, RR014758, RR003034, NS45012 and HG004436), an American Heart Association grant (09GRNT2300003) and in part by the Medical Research Service of the Department of Veterans Affairs. K.Z. was supported by National Institutes of Health (GM074913). We thank S.J. Kittner, R.A. Kittles, G. Newman and G.H. Gibbons for reading and discussing the manuscript, and the people who donated blood samples and members of the International HapMap project.
The authors declare no competing financial interests.
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