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A reprogrammable mouse strain from gene-targeted embryonic stem cells

Nature Methods volume 7, pages 5355 (2010) | Download Citation

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Abstract

The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.

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Acknowledgements

We thank G. Mostoslavsky (Boston University School of Medicine) for the STEMCCA construct and secondary viral MEFs, A. Foudi and S. Eminli for help with hematopoietic cell isolation and culture, A. Khalil for help in cloning the Collagen-OKSM targeting construct, J. Polo for help with cell culture as well as for inspiring discussions, and C. Konrad for advice on tumor histology. M.S. was supported by a postdoctoral fellowship from the Schering Foundation, and K.H. was supported by a US National Institutes of Health Director's Innovator Award as well as by funds provided by the Harvard Stem Cell Institute and Massachusetts General Hospital.

Author information

Affiliations

  1. Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, Boston, Massachusetts, USA.

    • Matthias Stadtfeld
    • , Nimet Maherali
    • , Marti Borkent
    •  & Konrad Hochedlinger
  2. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA.

    • Matthias Stadtfeld
    • , Nimet Maherali
    • , Marti Borkent
    •  & Konrad Hochedlinger
  3. Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.

    • Nimet Maherali
  4. Erasmus University Rotterdam, Rotterdam, The Netherlands.

    • Marti Borkent

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Contributions

M.S. and K.H. conceived the study; N.M. performed blastocyst injections; M.S. performed all other experiments with help from M.B.; M.S. and K.H. analyzed the data and wrote the manuscript.

Corresponding author

Correspondence to Konrad Hochedlinger.

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DOI

https://doi.org/10.1038/nmeth.1409

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