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'Injecting' yeast

Nature Methods volume 6, pages 513–514 (2009)Cite this article

Abstract

Yeast is a powerful genetic model system, but its rigid cell wall has prohibited microinjection. Using microfabricated channels to constrain the fission yeast Schizosaccharomyces pombe, we sheared local regions of individual cells with a piezoelectric unit. The cells remained viable, we detected actin patches in the cell after introduction of fluorescent phalloidin into the medium, and the cytokinetic ring was disrupted after injection of the myosin II inhibitor blebbistatin.

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Figure 1: Injecting a trapped yeast.
Figure 2: The myosin inhibitor blebbistatin destabilizes the cytokinetic ring.

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Acknowledgements

We thank M. Bady, A. Buguin, M. Heiman, J. Moseley, F. Kelly, F. Neumann, R. Norinsky and members of the Rockefeller University Laboratory Animal Research Center facility, A. North, N. Questembert-Balaban and E. Riveline for helpful suggestions, and P. Pérez (University of Salamanca) for providing strain PPG 5054. D.R. acknowledges grants from the CNRS, Association pour la Recherche sur le Cancer, Action Concertée Incitative Dynamique et Réactivité des Assemblages Biologiques, Vaincre la Mucoviscidose and the Rhône-Alpes region. This study was also supported by grants from the Breast Cancer Research Foundation and the Rockefeller University.

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Authors and Affiliations

  1. Laboratory of Yeast Genetics and Cell Biology, The Rockefeller University, New York, New York, USA

    Daniel Riveline & Paul Nurse

  2. Laboratoire de Spectrométrie Physique, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 5588, Université Joseph Fourier, Saint-Martin-d'Hères, France

    Daniel Riveline

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  1. Daniel Riveline
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Correspondence to Daniel Riveline.

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Supplementary Figures 1–4, Supplementary Table 1 (PDF 196 kb)

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Riveline, D., Nurse, P. 'Injecting' yeast. Nat Methods 6, 513–514 (2009). https://doi.org/10.1038/nmeth.1335

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