Abstract
Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.
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Acknowledgements
We thank T. Moore at Open Biosystems for production of library plasmid DNA. This work was supported by grant GM52486 to G.P. from the US National Institutes of Health, a National Institutes of Health Research Supplement to Promote Diversity in Health-Related Research to G.M.J., a Dean's Pilot Project grant from the Albert Einstein College of Medicine, and the Wellcome Trust.
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Contributions
G.M.J. performed experiments. T.C. and J.S., bioinformatics; S.H., clone management; A.W., sequencing. J.R. and I.D. directed and managed sequencing and bioinformatics; G.P. oversaw the project; G.M.J. and G.P. wrote the manuscript.
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G.P. and his lab receive compensation from Open Biosystems for the distribution of their library.
Supplementary information
Supplementary Text and Figures
Supplementary Figure 1, Supplementary Methods (PDF 698 kb)
Supplementary Table 1
Yeast Genomic Tiling Collection. (XLS 2214 kb)
Supplementary Table 2
YGPM plasmids that confer a sick or lethal phenotype. (XLS 15 kb)
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Jones, G., Stalker, J., Humphray, S. et al. A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae. Nat Methods 5, 239–241 (2008). https://doi.org/10.1038/nmeth.1181
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DOI: https://doi.org/10.1038/nmeth.1181
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