Subjects

Abstract

The psychoactive constituent of cannabis, Δ9-tetrahydrocannabinol, produces in humans subjective responses mediated by CB1 cannabinoid receptors, indicating that endogenous cannabinoids may contribute to the control of emotion. But the variable effects of Δ9-tetrahydrocannabinol obscure the interpretation of these results and limit the therapeutic potential of direct cannabinoid agonists. An alternative approach may be to develop drugs that amplify the effects of endogenous cannabinoids by preventing their inactivation. Here we describe a class of potent, selective and systemically active inhibitors of fatty acid amide hydrolase, the enzyme responsible for the degradation of the endogenous cannabinoid anandamide. Like clinically used anti-anxiety drugs, in rats the inhibitors exhibit benzodiazepine-like properties in the elevated zero-maze test and suppress isolation-induced vocalizations. These effects are accompanied by augmented brain levels of anandamide and are prevented by CB1 receptor blockade. Our results indicate that anandamide participates in the modulation of emotional states and point to fatty acid amide hydrolase inhibition as an innovative approach to anti-anxiety therapy.

Main

Anandamide, the naturally occurring amide of arachidonic acid with ethanolamine, meets all key criteria of an endogenous cannabinoid substance1: it is released on demand by stimulated neurons2,3; it activates cannabinoid receptors with high affinity1; and it is rapidly eliminated through a two-step process consisting of carrier-mediated transport followed by intracellular hydrolysis2,4. Anandamide hydrolysis is catalyzed by the enzyme fatty acid amide hydrolase (FAAH), a membrane-bound serine hydrolase5,6 that also cleaves other bioactive fatty acid ethanolamides such as oleoylethanolamide7 and palmitoylethanolamide8. Mutant mice lacking the gene encoding FAAH (Faah) cannot metabolize anandamide9 and, although fertile and generally normal, show signs of enhanced anandamide activity at cannabinoid receptors such as reduced pain sensation9. This is suggestive that drugs targeting FAAH may heighten the tonic actions of anandamide, while possibly avoiding the multiple and often unwanted effects produced by Δ9-tetrahydrocannabinol (Δ9-THC) and other direct-acting cannabinoid agonists10,11. To test this hypothesis, potent, selective and systemically active inhibitors of intracellular FAAH activity are needed. However, most current inhibitors of this enzyme lack the target selectivity and biological availability required for in vivo studies12,13,14, whereas newer compounds, though promising, have not yet been characterized15,16. Thus, the therapeutic potential of FAAH inhibition remains essentially unexplored.

Lead identification and optimization

Despite its unusual catalytic mechanism6, FAAH is blocked by a variety of serine hydrolase inhibitors, including compounds with activated carbonyls16. Therefore we examined whether esters of carbamic acid such as the anti-cholinesterase agent carbaryl (compound 1; Table 1) might inhibit FAAH activity in rat brain membranes. Although compound 1 was ineffective, its positional isomer 2 produced a weak inhibition of FAAH (half-maximal inhibitory concentration (IC50) = 18.6 ± 0.7 μM; mean ± s.e.m., n = 3), which was enhanced by replacing the N-methyl substituent with a cyclohexyl group (compound 3; IC50 = 324 ± 31 nM). The aryl ester 4, the benzyloxyphenyl group of which can be regarded as an elongated bioisosteric variant of the naphthyl moiety of compound 2, inhibited the activity of FAAH with a potency (IC50 = 396 ± 63 nM) equivalent to that of compound 3. A conformational analysis of compound 4 revealed families of accessible conformers differing mainly in the torsion angle around the O–CH2 bond, with substituents in anti or gauche conformations (data not shown). As the latter conformations more closely resembled the shape of the naphthyl derivative 3, we hypothesized that they might be responsible for the interaction of compound 4 with the active site of FAAH. Testing this hypothesis led to the design of the biphenyl derivative 5 (IC50 = 63 ± 9 nM), which was further optimized by systematic modifications of the distal phenyl group, resulting in the potent inhibitor 6 (IC50 = 4.6 ± 1.6 nM; Table 1). The lead optimization process will be reported elsewhere.

Table 1: Structures of selected carbamate inhibitors of FAAH activity

Kinetic analyses and dialysis experiments indicate that compounds 4 and 6 may inhibit FAAH activity through an irreversible interaction with the enzyme (data not shown), possibly due to a nucleophilic attack of an active serine residue on the carbamate group. This mechanism sets the present compounds apart from the α-keto heterocycle derivatives described previously16, which act as competitive FAAH inhibitors. A further indication of such a distinction is that in the α-keto heterocycle series potency is strongly dependent on the hydrophobicity of the flexible acyl chain, whereas in the carbamate series potency is modulated by the shape of the rigid aromatic moiety. Accordingly, when we replaced the biphenyl of compound 5 with a 5-phenylpentyl group, representing the most effective acyl chain in the α-keto heterocycle series, the inhibitory activity was lost (compound 7; Table 1).

Compounds 4 (URB532) and 6 (URB597) blocked the FAAH-catalyzed hydrolysis of exogenous [3H]anandamide in primary cultures of intact cortical neurons, with IC50 values that paralleled those obtained in membrane preparations (URB532, 214 ± 79 nM; URB597, 0.50 ± 0.05 nM; n = 8; Fig. 1a). By contrast, compound 8, an analog of URB532 that does not inhibit FAAH in membranes (Table 1), had no such effect (Fig. 1b). Moreover, URB532 and URB597 selectively impaired the breakdown of [3H]anandamide without reducing its carrier-mediated uptake, causing non-metabolized [3H]anandamide to accumulate in, and eventually exit from, the neurons. Thus, after a 4-minute incubation with [3H]anandamide, the intracellular content of non-metabolized [3H]anandamide was higher in inhibitor-treated neurons than in control neurons (Fig. 1c). As expected, the anandamide transport blocker N-(4-hydroxy- phenyl)arachidonamide (AM404) had an opposite effect, substantially reducing [3H]anandamide internalization4 (Fig. 1c). When neurons treated with URB597 were exposed for 4 minutes to [3H]anandamide and then incubated for 15 minutes in an [3H]anandamide-free solution, 42.6 ± 8.7% of the accumulated [3H]anandamide was released back into the medium (n = 3; Fig. 1d). This process was linear with time (Fig. 1e) and was not inhibited by AM404 (Fig. 1d), indicating that it occurred through passive diffusion rather than reverse transport. No such time-dependent release was observed in control neurons, the medium of which contained only residual levels of [3H]anandamide carried over from the pre-incubation period. These studies identify a new class of carbamate inhibitors of FAAH activity, which potently block anandamide breakdown in intact brain neurons.

Figure 1: The FAAH inhibitors URB532 and URB597 block [3H]anandamide degradation in intact brain neurons.
Figure 1

a, Concentration-dependent inhibition of [3H]anandamide hydrolysis by URB597 (open squares) and URB532 (filled circles) in primary cultures of rat cortical neurons. Baseline FAAH activity was 242.6 ± 3.9 cpm well min−1 (n = 4). b, Unlike URB532 (3 μM) or URB597 (10 nM), the inactive analog 8 (10 μM) has no effect on [3H]anandamide degradation. V, vehicle. c, URB532 (3 μM) and URB597 (10 nM) promote accumulation of non-metabolized [3H]anandamide in neurons, whereas the anandamide transport inhibitor AM404 (AM, 10 μM) reduces it. V, vehicle. d, Release of non-metabolized [3H]anandamide from neurons treated with URB597 (10 nM) during a 15-min incubation in the absence or presence of AM404 (AM, 10 μM). e, Time course of [3H]anandamide release from neurons treated with URB597 (10 nM). *P < 0.05; **P < 0.01 versus vehicle-treated neurons; ANOVA with Tukey's post-hoc test (n = 4–8). □, vehicle; ▪, treatments. Error bars, s.e.m.

Target selectivity

URB532 and URB597 inhibited FAAH, but did not affect the activities of three other serine hydrolases: electric eel acetylcholinesterase, horse plasma butyryl cholinesterase and rat brain monoglyceride lipase (MGL; Table 2). The lack of MGL inhibition is particularly noteworthy in light of the proposed role of this enzyme in the biological inactivation of 2-arachidonoylglycerol17 (2-AG), another endogenous cannabinoid present in brain18,19,20. Furthermore, URB532 and URB597 had no effect on anandamide transport in human astrocytoma cells or on the binding of a high-affinity ligand to CB1 and CB2 receptors (Table 2). In addition, URB532 (10 μM) did not significantly interact with a panel of 21 receptors, ion channels and neurotransmitter transporters, which included adenosine A1, A2A and A2B; adrenergic α1A, α2A, β1 and β2; dopamine D1 and D2; glutamate N-methyl-(D)-aspartate; γ-amino-butyric acid (GABA)A agonist site; histamine H1; opiate μ; muscarinic M2; and brain nicotinic receptors (data not shown). This high selectivity for FAAH encouraged us to examine the effects of URB532 and URB597 in live animals.

Table 2: Analysis of selected FAAH inhibitors in vitro

FAAH inhibition in vivo

Intraperitoneal (i.p.) injection of either URB532 or URB597, but not the inactive analog 8, produced a profound, dose-dependent inhibition of brain FAAH activity (Fig. 2a). In six experiments, half-maximal inhibition was reached at 0.60 ± 0.09 mg kg−1 of URB532, and 0.150 ± 0.007 mg kg−1 of URB597. The discrepancy between potency ratios of URB532 and URB597 in vitro (100-fold) and in vivo (4-fold) presumably reflects differences in bioavailability or brain penetration of the two compounds. After injection of a maximal dose of URB597 (0.3 mg kg−1, i.p.), FAAH inhibition was rapid in onset (<15 minutes), persistent (>6 hours; Fig. 2b) and accompanied by significant elevations in the brain levels of anandamide (Fig. 2c) and other fatty acid ethanolamides that are substrates for FAAH (in pmol g−1 of tissue at 2 hours after injection: oleoylethanolamide, vehicle, 137.0 ± 14.3, URB597 (0.3 mg kg−1), 725.3 ± 28.6; palmitoylethanolamide, vehicle, 259.1 ± 15.0, URB597, 1,324 ± 395; n = 8–15). Parallel changes in FAAH activity and fatty acid ethanolamide levels were also measured in various peripheral tissues (data not shown). In agreement with the lack of MGL inhibition noted in our in vitro experiments (Table 2), URB597 did not change the brain content of 2-AG (Fig. 2d).

Figure 2: In vivo inhibition of FAAH activity by URB532 and URB597.
Figure 2

a, Dose-dependent inhibition of brain FAAH activity by URB532 () and URB597 (□), but not by the inactive analog 8 (♦), after systemic (i.p.) administration in the rat. Baseline FAAH activity was 3,133 ± 59 cpm per mg of protein min−1 (n = 9). b, Time course of the inhibition of brain FAAH activity after a single injection of URB597 (0.3 mg kg−1, i.p.). c and d, Brain levels of anandamide (c) and 2-AG (d) 2 h after injections of vehicle (V) or URB597 (0.3 mg kg−1, i.p). *P < 0.05; **P < 0.01, ANOVA followed by Tukey's test; n = 4–8. e and f, Enhancement of anandamide-induced hypothermia by URB597. e, Time course of the effects of URB597 (0.3 mg kg−1, □), anandamide (5 mg kg−1, ; 5 mg kg−1, ) and anandamide (5 mg kg−1) plus URB597 (0.3 mg kg−1, 30 min before anandamide, ♦). Two-way ANOVA: Ftimes = 2.99, df = 12/416, P < 0.0005; Ftreatments = 52.25, df = 3/416, P < 0.0001; Ftimesxtreatments = 1.96, df = 36/416, P < 0.001. f, Effects of vehicle, URB597 (0.3 mg kg−1), anandamide (AEA, 5–30 mg kg−1), anandamide (5 mg kg−1) plus URB597 (0.3 mg kg−1), rimonabant (R, 1 mg kg−1), and anandamide (5 mg kg−1) plus URB597 (0.3 mg kg−1) and rimonabant (1 mg kg−1). One-way ANOVA: F = 27.22, df = 103, P < 0.0001. □, vehicle; ▪, urb597. Error bars, s.e.m.

As previously observed in Faah−/− mice9, FAAH inhibition was associated with increased sensitivity to the administration of exogenous anandamide. Accordingly, URB597 (0.3 mg kg−1, i.p.) intensified and prolonged the decrease in body temperature elicited by a subthreshold dose of anandamide (5 mg kg−1, i.p.), whereas it had no effect when injected alone (Figs. 2e and f). Underscoring the role of CB1 receptors in this response, the effect of anandamide plus URB597 was prevented by the CB1 antagonist SR141716A (rimonabant; Figs. 2e and f).

Pharmacological properties of FAAH inhibitors in vivo

Although URB532 and URB597 increased brain anandamide levels, they did not mimic the spectrum of pharmacological responses produced by exogenous anandamide. Systemic doses of URB532 (10 mg kg−1, i.p.) or URB597 (0.3 mg kg−1, i.p.) that maximally blocked FAAH activity produced no catalepsy (rigid immobility), hypothermia or hyperphagia (increased food intake), three typical signs of CB1 receptor activation11 (data not shown). However, the compounds exerted moderate anti-nociceptive actions in the mouse hot-plate test, which measures response to noxious thermal stimuli. In this model, URB532 and URB597 significantly lengthened response latencies at a dose of 0.5 mg kg−1 (Figs. 3a and b), but not 0.1 mg kg−1 (data not shown). These effects were prevented by a dose of the CB1 antagonist rimonabant (0.2 mg kg−1, intravenous (i.v.); Figs. 3a and b), which caused no hyperalgesia when administered alone (data not shown). Our results corroborate those obtained in Faah−/− mice9, indicating that acute disruption of FAAH activity results in a mild CB1-mediated anti-nociception, but no hypothermia or catalepsy.

Figure 3: Anti-nociceptive actions of URB532 and URB597.
Figure 3

Shown are effects of URB532 (a) and URB597 (b) (both at 0.5 mg kg−1, i.p.) on response latencies in the mouse hot-plate test, in the absence or presence of the CB1 antagonist rimonabant (Ri, 0.2 mg kg−1, i.v.). FAAH inhibitors and rimonabant were injected 60 min and 40 min before tests, respectively. Rimonabant alone had no effect on response latencies (not shown). *P < 0.05; ANOVA followed by Dunnett's test (n = 12). □, vehicle; ▪, urb597. Error bars, s.e.m.

Anxiolytic effects of FAAH inhibitors

To identify intrinsic actions of anandamide that might be significantly magnified by FAAH inhibition, we turned, for three reasons, to the regulation of emotional reactivity. First, CB1 receptors are expressed at high levels in brain regions such as the amygdala which are implicated in the control of anxiety and fear21,22,23. Second, acute administration of cannabinoid drugs produces emotional responses in rodents11 and humans10,24. Third, pharmacological25,26 or genetic27,45 thus, disruption of CB1 receptor activity elicits anxiety-like behaviors in rodents, suggestive of the existence of an intrinsic anxiolytic tone mediated by endogenous cannabinoids.

We used two pharmacologically validated animal models of anxiety: the elevated zero-maze test and the isolation-induced ultrasonic emission test. The zero maze consists of an elevated annular platform with two open and two closed quadrants and the test is based on the conflict between an animal's instinct to explore its environment and its fear of open spaces, where it may be attacked by predators28,29. Clinically used anxiolytic drugs such as the benzodiazepines increase the proportion of time spent in, and the number of entries made into, the open compartments. Similarly, URB532 (0.1–10 mg kg−1, i.p.) and URB597 (0.05–0.1 mg kg−1, i.p.) evoked anxiolytic-like responses at doses that corresponded to those required to inhibit FAAH activity in vivo (F = 24.7, df = 4/41, P < 0.001; F = 7.7, df = 2/27, P < 0.01; Figs. 4a and b). In keeping with an involvement of endogenous anandamide, the anxiolytic-like effects of both compounds were attenuated by a non-anxiogenic dose of rimonabant (2 mg kg−1, i.p.; F = 14.87, df = 3/28, P < 0.001; F = 15.2, df = 3/28, P < 0.001; Figs. 4c and d). Moreover, the effects were apparently dissociated from overall changes in motor behavior. Indeed, although URB532 elicited in adult rats a modest decrease in ambulation (which was also antagonized by rimonabant; data not shown), it did so at doses that were higher than those needed to cause anxiolysis (≥10 mg kg−1; F = 3.57, df = 2/22, P < 0.05; Fig. 4e). We confirmed this dissociation by testing URB532 and URB597 in the ultrasonic vocalization emission model, which measures the number of stress-induced vocalizations emitted by rat pups removed from their nest30,31,32. As seen with anxiolytic drugs, the FAAH inhibitors reduced ultrasonic calls (F = 10.8, df = 5/33, P < 0.001; F = 19.3; df = 4/25, P < 0.001; Figs. 4f and g) at doses (URB532, 1 and 5 mg kg−1; URB597, 0.1 mg kg−1) that had no effect on pup movement (data not shown). These anxiolytic-like responses were blocked by rimonabant (2 mg kg−1; Figs. 4f and g).

Figure 4: Anxiolytic-like actions of URB532 and URB597.
Figure 4

a and b, Dose-dependent effects of URB532 (a; 0.1–10 mg kg−1, i.p.) and URB597 (b; 0.05 and 0.1 mg kg−1, i.p.) on the percent of time spent by adult rats in the open quadrants of a zero maze (percent time open). c and d, Effect of the CB1 antagonist rimonabant (Ri, 2 mg kg−1, i.p.) on the change in percent time open produced by URB532 (c; 5 mg kg−1) and URB597 (d; 0.1 mg kg−1). e, Effects of URB532 (5 and 10 mg kg−1) on ambulation time in adult rats (20-min session). f and g, Effects of URB532 (f; 1–10 mg kg−1) and URB597 (g; 0.05–0.1 mg kg−1) on isolation-induced ultrasonic vocalizations in rat pups and blockade by rimonabant (2 mg kg−1, i.p.). *P < 0.05; **P < 0.01 (n = 6–10). □, vehicle; ▪, urb597. Error bars, s.e.m.

Discussion

We have developed a new class of agents that prevents anandamide inactivation by targeting the intracellular enzymatic activity of FAAH. URB597, the most potent member of this class, inhibited FAAH activity with an IC50 value of 4 nM in brain membranes and 0.5 nM in intact neurons, and an ID50 value of 0.15 mg kg−1 after systemic administration in the rat. This compound had much greater selectivity for FAAH than other cannabinoid-related targets, including cannabinoid receptors (selectivity index, >25,000) and MGL, an enzyme involved in the deactivation of the endogenous cannabinoid ester 2-AG (selectivity index, >7,500). Such target discrimination was matched by a lack of overt cannabimimetic effects in vivo. Thus, at doses that almost abolished FAAH activity and substantially raised brain anandamide levels, URB597 and its analog URB532 did not evoke catalepsy, reduce body temperature or stimulate feeding, three key symptoms of cannabinoid intoxication in the rodent11.

Nevertheless, the compounds did elicit marked anxiolytic-like responses, which paralleled their ability to inactivate FAAH and were prevented by the CB1 receptor antagonist rimonabant. We interpret these findings to indicate that URB597 and URB532 may selectively modulate anxiety-like behaviors by enhancing the tonic actions of anandamide on a subset of CB1 receptors which may normally be engaged in controlling emotions. Forebrain sites that might be implicated in such actions include the basolateral amygdala, the anterior cingulate cortex and the prefrontal cortex, all key elements of an “emotion circuit”33 that contains high densities of CB1 receptors21,22. CB1 receptors in these structures are localized to the axon terminals of a subpopulation of GABAergic interneurons, which also express the peptide cholecystokinin23,34 (CCK). The anxiogenic properties of CCK35 and the ability of CB1 agonists to inhibit K+-evoked CCK release from hippocampal slices36 indicate that interactions between this peptide and anandamide may participate in the control of anxiety.

In addition to their anxiolytic-like actions, URB597 and URB532 exerted modest anti-nociception in a model of acute pain, which also was sensitive to CB1 receptor blockade. These findings are similar to those reported for Faah−/− mice9 and support the proposed roles of anandamide in the intrinsic modulation of pain37. However, as emotional states may strongly influence pain sensation, it is possible that anxiolysis might have contributed to the mild anti-nociceptive effects of the FAAH inhibitors.

URB597 and URB532 increased brain anandamide levels without modifying those of the second endogenous cannabinoid, 2-AG. It is therefore likely that the pharmacological actions of these compounds, which are sensitive to the CB1 antagonist rimonabant, are primarily due to anandamide accumulation. But the FAAH inhibitors also produced large elevations in the levels of two anandamide analogs, palmitoylethanolamide and oleoylethanolamide, whose biological effects are independent of CB1 receptors7,8. Thus, we cannot exclude the possibility that additional properties of URB597 and URB532, mediated by these fatty ethanolamides, remain to be discovered.

Our results define a novel class of inhibitors of FAAH activity, which enhance endogenous anandamide signaling without directly interacting with cannabinoid receptors. The behavioral profile of these agents—characterized by anxiolysis and mild analgesia—reveals a key role for anandamide in the regulation of emotional states and indicates a new mechanistic approach to anti-anxiety therapy.

Methods

Animals and cells.

We used Wistar rats (200–350 g) and Swiss mice (20 g). All procedures met the National Institutes of Health guidelines for the care and use of laboratory animals and those of the Italian Ministry of Health (D.L. 116/92). We prepared cultures of cortical neurons from 18-day-old Wistar rat embryos and maintained them as described38. We purchased astrocytoma cells from American Type Culture Collection (Manassas, Virginia).

Chemicals.

Anandamide and related lipids were synthesized in the laboratory39. SR141716A (rimonabant) was provided by RBI (Natick, Massachusetts) as part of the Chemical Synthesis Program of the National Institutes of Health. AM404 was from Tocris (Avonmouth, UK) and other drugs from Sigma.

Synthesis of inhibitors.

n-Butylcarbamic acid 4-benzyloxyphenyl ester (URB532, compound 4) and 4-fluorophenylcarbamic acid 4-benzyloxyphenyl ester (compound 8) were obtained by treatment of 4-benzyloxyphenol with n-butylisocyanate and 4-fluorophenylisocyanate, respectively, with a catalytic amount of triethylamine in refluxing toluene. Similarly, cyclohexylcarbamic acid biphenyl-3-yl ester (compound 5), cyclohexylcarbamic acid 5-phenylpentyl ester (compound 7) and cyclohexylcarbamic acid 3′-carbamoylbiphenyl-3-yl ester (URB597; compound 6) were synthesized by reacting cyclohexylisocyanate with 3-phenylphenol, 5-phenylpentan-1-ol and 3′-hydroxybiphenyl-3-carboxylic acid amide, respectively. The latter reactant was prepared as follows: 3-bromobenzoic acid amide, obtained by reaction of 3-bromobenzonitrile and sodium perborate, was coupled with methoxyphenylboronic acid to give 3′-methoxybiphenyl-3-carboxylic acid amide, which was hydrolyzed with BBr3 to the desired 3′-hydroxybiphenyl-3-carboxylic acid amide. Detailed synthetic procedures and physicochemical data will be reported elsewhere.

Biochemical assays.

We prepared cell fractions from brain homogenates and assayed membrane FAAH activity and cytosol MGL activity using anandamide[ethanolamine-3H] (60 Ci/mmol; American Radiolabeled Chemicals (ARC), St. Louis, Missouri) and 2-mono-oleoyl-glycerol-[glycerol-1,2,3-3H] (20 Ci/mmol; ARC, St. Louis, Missouri), respectively, as substrates17. We conducted [3H]anandamide transport assays in human astrocytoma cells40; CB1 and CB2 binding assays in rat cerebellar membranes and CB2-overexpressing CHO cells (Receptor Biology-Perkin Elmer, Wellesley, Massachusetts), respectively, using [3H]WIN-55212-2 (NEN-Dupont, Boston, Massachusetts, 40-60 Ci/mmol) as a ligand1; and cholinesterase assays with a commercial kit (Sigma) using purified enzymes (electric eel acetylcholinesterase type V-S and horse serum cholinesterase; both from Sigma). To measure anandamide transport and hydrolysis in cortical neurons, we preincubated cells with FAAH inhibitors for 10 min at 37 °C, before exposure to [3H]anandamide for 4 min. In some experiments, we stopped the reactions with cold Tris-Krebs buffer containing 0.1% bovine serum albumin (Type V, fatty acid free, Sigma), removed the cells by trypsin-EDTA treatment and extracted lipids with chloroform/methanol (1/1, vol/vol). We measured non-metabolized [3H]anandamide in the organic phase and metabolized [3H]anandamide (as [3H]ethanolamine) in the aqueous phase. In other experiments, after having exposed the neurons to [3H]anandamide for 4 min, we rinsed the cells and measured [3H]anandamide release as described above.

High-performance liquid chromatography/mass spectrometry.

We extracted lipids with methanol-chloroform and fractionated them by column chromatography39. Anandamide and other fatty acid derivatives were quantified by high-performance liquid chromatography/mass spectrometry39.

Body temperature and catalepsy.

We administered compounds (in saline/Tween 80/polyethylene glycol, 90/5/5, i.p.) immediately before tests. We measured body temperature with a rectal probe connected to a digital thermometer (Physitemp Instruments, Clifton, New Jersey), and catalepsy as described41.

Food intake.

We administered URB597 (in DMSO/saline, 7:3, i.p.) 45 min before tests. We recorded food intake in free-feeding rats by using an automated system (Scipro Inc., New York, New York). After a 3-d acclimation, tests began at the onset of the dark phase and lasted for 24 h.

Anti-nociception.

FAAH inhibitors (in polyethylene glycol/water, 1:1) and rimonabant (in saline) were tested in the mouse hot-plate assay, as described4.

Anxiety and motor activity.

We dissolved FAAH inhibitors and rimonabant in dimethylsulfoxide (DMSO)/saline (7:3 and 9:1, respectively). We administered FAAH inhibitors by i.p. injection 30 min before tests and rimonabant 30 min before FAAH inhibitors. The elevated zero-maze apparatus is described elsewhere28,29. We placed the rats in a closed quadrant and video-recorded them for 5-min periods. Results are expressed as percent time in open quadrant/total time (percent time open). Results were analyzed by one-way ANOVA followed by Tukey's test. We recorded motor activity in an Opto-Varimex cage (Columbus Instruments, Columbus, Ohio) linked to a computer and placed in a sound-attenuated room illuminated by a 20-W white light. The amount of time spent in ambulatory activity was analyzed using an Auto-Track software42,43 (Columbus Instruments, Columbus, Ohio). Session duration was 20 min for adult rats and 60 s for 10-day-old pups. We analyzed data by overall one-way ANOVA followed by Tukey's test for individual between-group comparisons. We recorded 10-day-old pup ultrasonic vocalizations in a sound-attenuating chamber, as described44. Tests were conducted between 900 and 1400 h and lasted for 15 s. Drugs were administered after baseline value collection (15 s) and pups were tested again 30 min after drug administration. Data were expressed as percent change from baseline and analyzed by overall one-way ANOVA followed by Tukey's test for individual between-group comparisons.

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Acknowledgements

We thank A. Giuffrida and M. Solbrig for critical reading of the manuscript; B. Ferrer and F. Rodríguez de Fonseca for help with initial experiments; and J. Yoo and C. Park for technical assistance. The assistance of the Centro Interfacoltà Misure (CIM) and the Centro di Calcolo at the University of Parma is gratefully acknowledged. This research was supported by grants from the National Institute on Drug Abuse (to D.P.) and from MIUR, CNR and Universities of Parma and Urbino. D.P. is a Staglin Music Festival Investigator of the National Alliance for Research on Schizophrenia and Depression.

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  1. Department of Pharmacology, University of California, Irvine, California, USA

    • Satish Kathuria
    • , Silvana Gaetani
    • , Darren Fegley
    • , Fernando Valiño
    •  & Daniele Piomelli
  2. Institute of Medicinal Chemistry, University of Urbino, Urbino, Italy

    • Andrea Duranti
    • , Andrea Tontini
    •  & Giorgio Tarzia
  3. Pharmaceutical Department, University of Parma, Parma, Italy

    • Marco Mor
  4. Department of Experimental Pharmacology, University of Naples, Naples, Italy

    • Giovanna La Rana
    •  & Antonio Calignano
  5. Department of Pharmacology and Human Physiology, University of Bari, Bari, Italy

    • Arcangela Giustino
    •  & Maria Tattoli
  6. Department of Pharmacology and General Physiology, University of Rome “La Sapienza”, Rome, Italy

    • Maura Palmery
    •  & Vincenzo Cuomo

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Competing interests

A patent has been filed, with A.D., A.T., M.M., G.T. and D.P. listed as inventors, the value of which may be affected by the publication of this work.

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Correspondence to Daniele Piomelli.

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https://doi.org/10.1038/nm803

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