Cell transplantation with embryonic stem (ES) cell progeny requires immunological compatibility with host tissue. 'Therapeutic cloning' is a strategy to overcome this limitation by generating nuclear transfer (nt)ES cells that are genetically matched to an individual. Here we establish the feasibility of treating individual mice via therapeutic cloning. Derivation of 187 ntES cell lines from 24 parkinsonian mice, dopaminergic differentiation, and transplantation into individually matched host mice showed therapeutic efficacy and lack of immunological response.
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We thank J.P.H. Burb ach (Rudoplph Magnus Institute, Utrecht), M. Smidt (Rudolph Magnus Institute, Utrecht), K. Rajewsky (The CBD Institute, Massachusetts), C. Lobe (University of Toronto, Canada) and M.A.S. Moore (Sloan-Kettering Institute, New York) for reagents, A.C.F. Perry for helpful discussions and M. Leversha for technical assistance. Supported by the US National Institute of Neurological Disorders and Stroke (R21NS44231 and R01NS052671), the Starr Tri-institutional Stem Cell Initiative, the Michael J. Fox Foundation for Parkinson's Research, the Michael W. McCarthy Foundation and an unrestricted grant from the Kinetics Foundation.
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Tabar, V., Tomishima, M., Panagiotakos, G. et al. Therapeutic cloning in individual parkinsonian mice. Nat Med 14, 379–381 (2008). https://doi.org/10.1038/nm1732
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