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Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration

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Figure 1: The pTAT-HA vector and purification protocol.
Figure 2: Analysis of transduced proteins.
Figure 3: In vivo biochemical and biological properties of transduced proteins.
Figure 4: Induction of cell scattering and filopodia by TAT-p27wt protein.

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Acknowledgements

We thank J. Roberts for wild-type p27 cDNA, J. Massague for p27 kkpv mutant cDNA; E. Harlow for Cdk2-DN cDNA; M. Dustin for confocal microscopy; and all members of the Dowdy lab for critical input. This work was supported by an NIH-MSTP fellowship (E.L.S.) and the Howard Hughes Medical Institute (S.F.D.).

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Correspondence to Steven F. Dowdy.

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Nagahara, H., Vocero-Akbani, A., Snyder, E. et al. Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration. Nat Med 4, 1449–1452 (1998). https://doi.org/10.1038/4042

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