Bone destruction is a pathological hallmark of several chronic inflammatory diseases, including rheumatoid arthritis and periodontitis. Inflammation-induced bone loss of this sort results from elevated numbers of bone-resorbing osteoclasts. Gene targeting studies have shown that the transcription factor nuclear factor-κB (NF-κB) has a crucial role in osteoclast differentiation, and blocking NF-κB is a potential strategy for preventing inflammatory bone resorption. We tested this approach using a cell-permeable peptide inhibitor of the IκB-kinase complex, a crucial component of signal transduction pathways to NF-κB. The peptide inhibited RANKL-stimulated NF-κB activation and osteoclastogenesis both in vitro and in vivo. In addition, this peptide significantly reduced the severity of collagen-induced arthritis in mice by reducing levels of tumor necrosis factor-α and interleukin-1β, abrogating joint swelling and reducing destruction of bone and cartilage. Therefore, selective inhibition of NF-κB activation offers an effective therapeutic approach for inhibiting chronic inflammatory diseases involving bone resorption.
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This work was supported by the National Institutes of Health (R37-AI33443) and the Howard Hughes Medical Institute (S.G.), a Scientist Development Grant from the American Heart Association (M.J.M.), by Grants-in-Aid for Scientific Research (13557151) from the Ministry of Education, Culture, Sports Science and Technology of Japan (K.A.) and by the Takeda Science Foundation (E.J.).
The authors declare no competing financial interests.
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Jimi, E., Aoki, K., Saito, H. et al. Selective inhibition of NF-κB blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo. Nat Med 10, 617–624 (2004). https://doi.org/10.1038/nm1054
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