Generating mice with targeted mutations

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Figure 1: Regenera-tion of a functional neor by gene targeting.
Figure 2: Disruption of Hprt by gene targeting.
Figure 3: The positive–negative selection procedure used to enrich for ES cells containing a targeted disruption of gene X.
Figure 4: Generation of mouse germline chimeras from ES cells containing a targeted mutation.

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Capecchi, M. Generating mice with targeted mutations. Nat Med 7, 1086–1090 (2001). https://doi.org/10.1038/nm1001-1086

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