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DNA immunization induces protective immunity against B–cell lymphoma

Nature Medicine volume 2, pages 1038–1041 (1996)Cite this article

Abstract

Idiotypic determinants of the immunoglobuiin expressed on the surface of B–cell lymphomas are tumor–specific antigens (TSAs), which can be targeted by immunotherapy. Immunization with DNA constructs encoding the idiotype (Id) of a murine B–cell lymphoma induced specific anti–Id antibody responses and protected mice against tumor challenge. Use of DNA encoding an Id/GM–CSF (idiotype/granulocyte–macrophage colony–stimulating factor) fusion protein improved vaccine efficacy, and xenogeneic immunoglobuiin constant region determinants were required for immunogenicity. These results indicate that DNA may be a simple and efficacious means of inducing immune responses against a weak, otherwise unrecognized tumor antigen, provided that additional stimuli are included with the DNA.

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Authors and Affiliations

  1. Department of Medicine, Division of Oncology, Stanford University School of Medicine, Stanford University Medical Center, Room M207, Stanford, California, 94305-5306, USA

    Athanasia D. Syrengelas, Thomas T. Chen & Ronald Levy

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  1. Athanasia D. Syrengelas
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  2. Thomas T. Chen
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  3. Ronald Levy
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Syrengelas, A., Chen, T. & Levy, R. DNA immunization induces protective immunity against B–cell lymphoma. Nat Med 2, 1038–1041 (1996). https://doi.org/10.1038/nm0996-1038

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