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T-cell epitope mapping by flow cytometry

Nature Medicine volume 4, pages 975–978 (1998)Cite this article

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References

  1. Moss, P.A. et al. Persistent high frequency of human immunodeficiency virus-specific cytotoxic T cells in peripheral blood of infected donors. Proc. Natl. Acad. Sci. USA 92, 5773–5777 (1995).

    Article  CAS  Google Scholar 

  2. Borysiewicz, L.K. et al. Human cytomegalovirus-specific cytotoxic T cells. Relative frequency of stage-specific CTL recognizing the 72-kD immediate early protein and glycoprotein B expressed by recombinant vaccinia viruses. J. Exp. Med. 168, 919–931 (1988).

    Article  CAS  Google Scholar 

  3. Gross, H.J., Verwer, B., Houck, D. & Recktenwald, D. Detection of rare cells at a frequency of one per million by flow cytometry. Cytometry 14, 519–526 (1993).

    Article  CAS  Google Scholar 

  4. Radbruch, A. & Recktenwald, D. Detection and isolation of rare cells. Curr. Opin. Immunol. 7, 270–273 (1995).

    Article  CAS  Google Scholar 

  5. Waldrop, S.L., Pitcher, C.J., Peterson, D.M., Maino, V.C. & Picker, L.J. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: Evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J. Clin. Invest. 99, 1739–1750 (1997).

    Article  CAS  Google Scholar 

  6. Wills, M.R. et al. The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: Frequency, specificity, and T-cell receptor usage of pp65-specific CTL. J. Virol. 70, 7569–7579 (1996).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Rammensee, H.G., Friede, T. & Stefanovic, S. MHC ligands and peptide motifs: First listing. Immunogenetics 41, 178–228 (1995).

    Article  CAS  Google Scholar 

  8. Diamond, D.J., York, J., Sun, J.Y., Wright, C.L. & Forman, S.J. Development of a candidate HLA A*0201 restricted peptide-based vaccine against human cytomegalovirus infection. Blood 90, 1751–1767 (1997).

    CAS  PubMed  Google Scholar 

  9. Parker, K.C., Bednarek, M.A. & Coligan, J.E. Scheme for ranking potential HLA-A2 binding peptides based on independent binding of individual peptide side-chains. J. Immunol. 152, 163–175 (1996).

    Google Scholar 

  10. Sherman, L.A., Burke, T.A. & Biggs, J.A. Extracellular processing of peptide antigens that bind class I major histocompatibility molecules. J. Exp. Med. 175, 1221–1226 (1992).

    Article  CAS  Google Scholar 

  11. Rammensee, H.G., Bachmann, J. & Stevanovic, S. in. MHC Ligands and Peptide Motifs. (Landes Bioscience, Georgetown, Texas, 1997).

    Book  Google Scholar 

  12. Brusic, V., Rudy, G., Harrison, L.C. MHCPEP, a database of MHC-binding peptides: Update 1997. Nucleic Acids Res. 26, 370–373 (1998).

    Article  Google Scholar 

  13. Roederer, M. et al. 8 color, 10-parameter flow cytometry to elucidate complex leukocyte heterogeneity. Cytometry 29, 328–339 (1997).

    Article  CAS  Google Scholar 

  14. Assenmacher, M. et al. Specific expression of surface interferon-gamma on interferon-gamma producing T cells from mouse and man. Eur. J. Immunol. 26, 263–267 (1996).

    Article  CAS  Google Scholar 

Download references

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Author notes

  1. Florian Kern: Correspondence should be addressed to F.K.

Authors and Affiliations

  1. Institut für Medizinische Immunologie, Medizinische Fakultät der Humboldt-Universität zu Berlin, Campus Mitte, 10098, Berlin, Germany

    Florian Kern, Ingolf Pascal Surel, Jens Schneider-Mergener & Hans-Dieter Volk

  2. Dermatologische Klinik, Medizinische Fakultät der Humboldt-Universität zu Berlin, Campus Mitte, 10098, Berlin, Germany

    Carsten Brock & Peter Walden

  3. Institut für Transfusionsmedizin und Immunhämatologie, Medizinische Fakultät der Humboldt-Universität zu Berlin, Campus Mitte, 10098, Berlin, Germany

    Bernd Freistedt & Hartmut Radtke

  4. Deutsches Rheuma Forschungs-Zentrum (DRFZ) Berlin, Hannoversche Straβe 27, 10115, Berlin, Germany

    Alexander Scheffold & Andreas Radbruch

  5. Medizinische Klinik mit Schwerpunkt Hämatologie und Onkologie, Germany

    Rainer Blasczyk

  6. Medizinische Klinik mit Schwerpunkt Nephrologie und Intensivmedizin, Medizinische Fakultät der Humboldt-Universität zu Berlin, Campus Virchow, Augustenburger Platz 1, 13353, Berlin, Germany

    Petra Reinke

Authors
  1. Florian Kern
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  2. Ingolf Pascal Surel
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  3. Carsten Brock
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  4. Bernd Freistedt
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  5. Hartmut Radtke
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  6. Alexander Scheffold
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  7. Rainer Blasczyk
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  8. Petra Reinke
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  9. Jens Schneider-Mergener
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  10. Andreas Radbruch
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  11. Peter Walden
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  12. Hans-Dieter Volk
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Kern, F., Surel, I., Brock, C. et al. T-cell epitope mapping by flow cytometry. Nat Med 4, 975–978 (1998). https://doi.org/10.1038/nm0898-975

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