The adult heart lacks reserve cardiocytes and cannot regenerate. Therefore, a large acute myocardial infarction often develops into congestive heart failure. To attempt to prevent this progression, we transplanted skeletal myoblasts into cryoinfarcted myocardium of the same rabbits (autologous transfer), monitored cardiac function in vivo for two to six weeks and examined serial sections of the hearts by light and electron microscopy. Islands of different sizes comprising elongated, striated cells that retained characteristics of both skeletal and cardiac cells were found in the cryoinfarct. In rabbits in which myoblasts were incorporated, myocardial performance was improved. The ability to regeneratefunctioning muscle after autologous myoblast transplantation could have a important effect on patients after acute myocardial infarction.
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Taylor, D.A. et al. Delivery of Primary Autologous Skeletal Myoblasts into Rabbit Heart by Coronary Infusion: A Potential Approach to Myocardial Repair. Proc. Assoc. Am. Physicians 109, 245–253 (1997).
Robinson, S.W. et al. Arterial delivery of genetically labelled skeletal myoblasts to the murine heart: long-term survival and phenotypic modification of implanted myoblasts. Cell Transplant. 5, 77–91 (1996).
Chiu, R.C.-J., Zibaitis, A. & Kao, R.L. Cellular cardiomyoplasty: myocardial regeneration with satellite cell implantation. Ann. Thorac. Surg. 60, 12–18 (1995).
Marelli, D., Desrosiers, C., El-Alfy, M., Kao, R.L. & Chiu, R.C.-J. Cell transplantation for myocardial repair: an experimental approach. Cell Transpant. 1, 383–390 (1992).
Soonpaa, M.H., Koh, C.Y., Klug, M.G. & Field, L.G. Formation of nascent intercalated disks between grafted cardiomyocytes and host myocardium. Science 264, 98–101 (1994).
Koh, G.Y., Soonpaa, M.H., Klug, M.G. & Field, L.J. Long-term survival of AT-1 cardiomyocyte grafts in syngeneic myocardium. Am. J. Physiol. 264 (Heart Circ Physiol 33), H1727–H1733 (1993).
Klung, M., Soonpaa, M., Koh, G. & Field, L. Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts. J. Clin. Invest. 98, 216–224 (1996).
Bischoff, R. Regeneration of single skeletal muscle fibers in vitro. Anat. Rec. 182, 215–236 (1975).
Watt, D.J., Karasinski, J., Moss, J. & England, M.A. Migration of muscle cells. Nature 368, 406–408 (1994).
Stockdale, F.E., Hager, E.J., Fernyak, S.E. & DiMario, J.X. in Myoblast Transfer Therapy (eds. Criggs, R.C. & Karparti, G.) 7–11 (Plenum Press, New York, 1990).
Drake-Holland, A.J., Belcher, P., Hynd, J. & Noble, M.I.M. Infarct size in rabbits: a modified method illustrated by the effects of propranolol and trimetazidine. Basic Res. Cardiol. 88, 250–258 (1993).
Gill, W., Da Costa, J. & Fraser, J. The control and predictability of a cryolesion. Cryobiol. 6, 347–353 (1970).
Gill, W. & Long, W.B. The completeness of cellular destruction within a cryolesion. Brit. J. Surg. 58, 870 (1971).
Glower, D.D. et al. Linearity of the Frank-Starling relationship in the intact heart: the concept of preload recruitable stroke work. Circulation 1985, 994–1009 (1985).
Silvestry, S.C. et al. The in vivo quantification of myocardial performance in rabbits: a model for evaluation of cardiac gene therapy. J. Mol. Cell. Cardiol. 28, 815–823 (1996).
Glantz, S.A. & Parmley, W.W. Factors which affect the diastolic pressure-volume curve. Circ. Res. 42, 171–180 (1978).
Magid, N.M. et al. Left ventricular diastolic and systolic performance during chronic aortic regurgitation. Am. J. Physiol. 263, H226–H233 (1992).
Guerette, B., Asselin, I., Skuk, D., Entman, M. & Tremblay, J.P. Control of inflammatory damage by anti-LFA-1: increase success of myoblast transplantation. Cell Transpl. 6, 101–107 (1997).
Jarvis, J.C., Sutherland, H., Kwende, M.M.N., Mayne, C.N. & Salmons, S. in Cardiac Bioassist (eds. Carpentier, A., Chachques, J.C. & Grandjean, P.) 269–272 (Futura Publishing, Armonk, NY, 1997).
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Taylor, D., Atkins, B., Hungspreugs, P. et al. Regenerating functional myocardium: Improved performance after skeletal myoblast transplantation. Nat Med 4, 929–933 (1998). https://doi.org/10.1038/nm0898-929
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