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Regenerating functional myocardium: Improved performance after skeletal myoblast transplantation

Nature Medicine volume 4, pages 929–933 (1998)Cite this article

An Erratum to this article was published on 01 October 1998

Abstract

The adult heart lacks reserve cardiocytes and cannot regenerate. Therefore, a large acute myocardial infarction often develops into congestive heart failure. To attempt to prevent this progression, we transplanted skeletal myoblasts into cryoinfarcted myocardium of the same rabbits (autologous transfer), monitored cardiac function in vivo for two to six weeks and examined serial sections of the hearts by light and electron microscopy. Islands of different sizes comprising elongated, striated cells that retained characteristics of both skeletal and cardiac cells were found in the cryoinfarct. In rabbits in which myoblasts were incorporated, myocardial performance was improved. The ability to regeneratefunctioning muscle after autologous myoblast transplantation could have a important effect on patients after acute myocardial infarction.

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References

  1. Taylor, D.A. et al. Delivery of Primary Autologous Skeletal Myoblasts into Rabbit Heart by Coronary Infusion: A Potential Approach to Myocardial Repair. Proc. Assoc. Am. Physicians 109, 245–253 (1997).

    CAS  PubMed  Google Scholar 

  2. Robinson, S.W. et al. Arterial delivery of genetically labelled skeletal myoblasts to the murine heart: long-term survival and phenotypic modification of implanted myoblasts. Cell Transplant. 5, 77–91 (1996).

    Article  CAS  Google Scholar 

  3. Chiu, R.C.-J., Zibaitis, A. & Kao, R.L. Cellular cardiomyoplasty: myocardial regeneration with satellite cell implantation. Ann. Thorac. Surg. 60, 12–18 (1995).

    Article  CAS  Google Scholar 

  4. Marelli, D., Desrosiers, C., El-Alfy, M., Kao, R.L. & Chiu, R.C.-J. Cell transplantation for myocardial repair: an experimental approach. Cell Transpant. 1, 383–390 (1992).

    Article  CAS  Google Scholar 

  5. Soonpaa, M.H., Koh, C.Y., Klug, M.G. & Field, L.G. Formation of nascent intercalated disks between grafted cardiomyocytes and host myocardium. Science 264, 98–101 (1994).

    Article  CAS  Google Scholar 

  6. Koh, G.Y., Soonpaa, M.H., Klug, M.G. & Field, L.J. Long-term survival of AT-1 cardiomyocyte grafts in syngeneic myocardium. Am. J. Physiol. 264 (Heart Circ Physiol 33), H1727–H1733 (1993).

    CAS  PubMed  Google Scholar 

  7. Klung, M., Soonpaa, M., Koh, G. & Field, L. Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts. J. Clin. Invest. 98, 216–224 (1996).

    Article  Google Scholar 

  8. Bischoff, R. Regeneration of single skeletal muscle fibers in vitro. Anat. Rec. 182, 215–236 (1975).

    Article  CAS  Google Scholar 

  9. Watt, D.J., Karasinski, J., Moss, J. & England, M.A. Migration of muscle cells. Nature 368, 406–408 (1994).

    Article  CAS  Google Scholar 

  10. Stockdale, F.E., Hager, E.J., Fernyak, S.E. & DiMario, J.X. in Myoblast Transfer Therapy (eds. Criggs, R.C. & Karparti, G.) 7–11 (Plenum Press, New York, 1990).

    Book  Google Scholar 

  11. Drake-Holland, A.J., Belcher, P., Hynd, J. & Noble, M.I.M. Infarct size in rabbits: a modified method illustrated by the effects of propranolol and trimetazidine. Basic Res. Cardiol. 88, 250–258 (1993).

    CAS  PubMed  Google Scholar 

  12. Gill, W., Da Costa, J. & Fraser, J. The control and predictability of a cryolesion. Cryobiol. 6, 347–353 (1970).

    Article  CAS  Google Scholar 

  13. Gill, W. & Long, W.B. The completeness of cellular destruction within a cryolesion. Brit. J. Surg. 58, 870 (1971).

  14. Glower, D.D. et al. Linearity of the Frank-Starling relationship in the intact heart: the concept of preload recruitable stroke work. Circulation 1985, 994–1009 (1985).

    Article  Google Scholar 

  15. Silvestry, S.C. et al. The in vivo quantification of myocardial performance in rabbits: a model for evaluation of cardiac gene therapy. J. Mol. Cell. Cardiol. 28, 815–823 (1996).

    Article  CAS  Google Scholar 

  16. Glantz, S.A. & Parmley, W.W. Factors which affect the diastolic pressure-volume curve. Circ. Res. 42, 171–180 (1978).

    Article  CAS  Google Scholar 

  17. Magid, N.M. et al. Left ventricular diastolic and systolic performance during chronic aortic regurgitation. Am. J. Physiol. 263, H226–H233 (1992).

    Article  CAS  Google Scholar 

  18. Guerette, B., Asselin, I., Skuk, D., Entman, M. & Tremblay, J.P. Control of inflammatory damage by anti-LFA-1: increase success of myoblast transplantation. Cell Transpl. 6, 101–107 (1997).

    CAS  Google Scholar 

  19. Jarvis, J.C., Sutherland, H., Kwende, M.M.N., Mayne, C.N. & Salmons, S. in Cardiac Bioassist (eds. Carpentier, A., Chachques, J.C. & Grandjean, P.) 269–272 (Futura Publishing, Armonk, NY, 1997).

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Duke University Medical Center, PO Box 3327, Durham, North Carolina, 27710, USA

    Doris A. Taylor, Kelley A. Hutcheson & William E. Kraus

  2. Department of Surgery, Duke University Medical Center, PO Box 3851, Durham, North Carolina, 27710, USA

    Doris A. Taylor, B. Zane Atkins & Donald D. Glower

  3. Department of Biomedical Engineering, Duke University Medical Center, PO Box 3327, Durham, North Carolina, 27710, USA

    Doris A. Taylor, Pinata Hungspreugs, Thomas R. Jones & Donald D. Glower

  4. Department of Cell Biology, Duke University Medical Center, PO Box 3327, Durham, North Carolina, 27710, USA

    Mary C. Reedy & William E. Kraus

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  1. Doris A. Taylor
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Taylor, D., Atkins, B., Hungspreugs, P. et al. Regenerating functional myocardium: Improved performance after skeletal myoblast transplantation. Nat Med 4, 929–933 (1998). https://doi.org/10.1038/nm0898-929

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