The transfer of apoptosis genes to tumors is one of the most promising strategies for cancer gene therapy. We have shown that massive apoptosis occurs when wild-type p53 expression is induced in glioma cells carrying a p53 gene mutation. However, adenovirus-mediated p53 gene transfer is ineffective in causing apoptosis in glioma cells that retain a wild-type p53 genotype. We evaluated the effect of E2F-1 overexpression on the growth of gliomas in vitro and in vivo. In the in vitro study, the adenovirus-mediated transfer of exogenous E2F-1 protein precipitated generalized apoptosis in gliomas. The treatment with AdSCMV-f 2F-1 of nude mice carrying subcutaneous gliomas arrested tumor growth. Our results indicate that E2F-T has anti-glioma activity in vitro and in vivo.
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Fueyo, J., Gomez-Manzano, C., Yung, W. et al. Overexpression of E2F‐1 in glioma triggers apoptosis and suppresses tumor growth in vitro and in vivo. Nat Med 4, 685–690 (1998) doi:10.1038/nm0698-685
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