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Ribozyme trans–splicing and RNA tagging: Following the messenger

Splicing ribozymes can be used to repair mutant RNA transcripts in mammalian cells (pages 643–648).

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References

  1. Cech, T.R. Ribozymes and their medical implications. J. Am. Med. Assoc. 260, 3030–3034 (1988).

    Article  CAS  Google Scholar 

  2. Castanotto, D., Rossi, J.J. & Sarver, N. Antisense catalytic RNAs as therapeutic agents. Adv. Pharmacol. 25, 289–317 (1994).

    Article  CAS  Google Scholar 

  3. Zaug, A.J., Been, M.D. & Cech, T.R. The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature 324, 429–433 (1986).

    Article  CAS  Google Scholar 

  4. Haseloff, J. & Gerlach, W.L. Simple RNA enzymes with new and highly specific endonuclease activities. Nature 334, 585–591 (1988).

    Article  CAS  Google Scholar 

  5. Sarver, N. et al. Ribozymes as potential anti-HIV-1 therapeutic agents. Science 247, 1222–1225 (1990).

    Article  CAS  Google Scholar 

  6. Sullenger, B.A. & Cech, T.R. Ribozyme-mediated repair of defective mRNA by targeted trans-splicing. Nature 371, 619–622 (1994).

    Article  CAS  Google Scholar 

  7. Cech, T.R. Self splicing of group I introns. Ann. Rev. Biochem. 59, 543–568 (1990).

    Article  CAS  Google Scholar 

  8. Inoue, T., Sullivan, F.X. & Cech, T.R. Inter-molecular exon ligation of the tRNA precursor of Tetrahymena oligonucleotides can function as 5′ exons. Cell 43, 431–437 (1985).

    Article  CAS  Google Scholar 

  9. Been, M.D. & Cech, T.R. The binding site determines sequence specificity of Tetrahymena pre-mRNA self splicing, trans-splicing, and RNA enzyme activity. Cell 47, 207–216 (1986).

    Article  CAS  Google Scholar 

  10. Jones, J.T., Lee, S-W. & Sullenger, B.A. Tagging ribozymes reaction sites to follow trans-splicing in mammalian cells. Nature Med. 2, 643–648 (1996).

    Article  CAS  Google Scholar 

  11. Young, B., Herschlag, D. & Cech, T.R. Mutations in a nonconserved sequence of the Tetrahymena ribozyme increase activity and specificity. Cell 67, 1007–1019 (1991).

    Article  CAS  Google Scholar 

  12. Pyle, A.M., Murphy, F.L. & Cech, T.R. RNA substrate binding sites in the catalytic core of the Tetrahymena ribozyme. Nature 358, 123–128 (1992).

    Article  CAS  Google Scholar 

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Sarver, N., Cairns, S. Ribozyme trans–splicing and RNA tagging: Following the messenger. Nat Med 2, 641–642 (1996). https://doi.org/10.1038/nm0696-641

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