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p53 activates expression of HIC-1, a new candidate tumour suppressor gene on 17p13.3

Nature Medicine volume 1, pages 570–577 (1995)Cite this article

Abstract

For several human tumour types, allelic toss data suggest that one or more tumour suppressor genes reside telomeric to the p53 gene at chromosome 17p13.1. In the present study we have used a new strategy, involving molecular analysis of a DNA site hypermethylated in tumour DNA, to identify a candidate gene in this region (17p13.3). Our approach has led to identification of HIC-1 (hypermethylated in cancer), a new zinc-finger transcription factor gene which is ubiquitously expressed in normal tissues, but underexpressed in different tumour cells where it is hypermethylated. Multiple characteristics of this gene, including the presence of a p53 binding site in the 5′ flanking region, activation of the gene by expression of a wild-type p53 gene and suppression of C418 selectability of cultured brain, breast and colon cancer cells following insertion of the gene, make HIC-1 gene a strong candidate for a tumour suppressor gene in region 17p13.3.

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Author information

Author notes

  1. Wafik El Deiry

    Present address: Hematology-Oncology Division, University of Pennsylvania School of Medicine, 36th and Hamilton Walk, Philadelphia, Pennsylvania, 19104, USA

Authors and Affiliations

  1. Human Genetics Graduate Program, 424 North Bond Street, Baltimore, Maryland, 21231, USA

    Michele Makos Wales, Margaret A. Biel & Stephen B. Baylin

  2. The Oncology Center, the Johns Hopkins Medical Institutions, 424 North Bond Street, Baltimore, Maryland, 21231, USA

    Wafik El Deiry, Barry D. Nelkin, Jean-Pierre Issa, Steven J. Kuerbitz & Stephen B. Baylin

  3. The Ludwig Cancer Institute for Cancer Research and Department of Medicine and Center for Molecular Genetics, University of California at San Diego, La Jolla, California, 92093, USA

    Webster K. Cavenee

  4. Department of Pediatrics, the Johns Hopkins Medical Institutions, 720 Rutland Avenue, Baltimore, Maryland, 21205, USA

    Steven J. Kuerbitz

  5. Department of Medicine, the Johns Hopkins Medical Institutions, 720 Rutland Avenue, Baltimore, Maryland, 21205, USA

    Stephen B. Baylin

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Wales, M., Biel, M., Deiry, W. et al. p53 activates expression of HIC-1, a new candidate tumour suppressor gene on 17p13.3. Nat Med 1, 570–577 (1995). https://doi.org/10.1038/nm0695-570

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