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Induction of human autologous cytotoxic T lymphocytes on formalin-fixed and paraffin-embedded tumour sections

Nature Medicine volume 1, pages 267–271 (1995)Cite this article

An Erratum to this article was published on 01 April 1995

Abstract

Human autologous tumour-specific cytotoxic T lymphocytes (CTL) were generated from peripheral blood on small formalin-fixed paraffin-embedded sections of a gastric cancer. The CTL killed live target cells at an effector/target ratio of 1 within 24 hours and showed the same target specificity as those induced on live cancer cells. The killing activity of the CTL lasted for more than four months in culture and was inhibited by antibodies against CD8 and MHC-class I. These results suggest that adoptive immunotherapy of tumours will be possible with CTL induced on a stable source of tumour antigen.

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Author notes

  1. Tadao Ohno: Correspondence should be addressed to T.O.

Authors and Affiliations

  1. RIKEN Cell Bank, The Institute of Physical and Chemical Research, University of Tsukuba, Tsukuba Science City, Ibaraki, 305, Japan

    Shu Qin Liu, Kaoru Saijo & Tadao Ohno

  2. The Department of Surgery, Medical School, University of Tsukuba, Tsukuba Science City, Ibaraki, 305, Japan

    Takeshi Todoroki

Authors
  1. Shu Qin Liu
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  2. Kaoru Saijo
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  3. Takeshi Todoroki
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  4. Tadao Ohno
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Liu, S., Saijo, K., Todoroki, T. et al. Induction of human autologous cytotoxic T lymphocytes on formalin-fixed and paraffin-embedded tumour sections. Nat Med 1, 267–271 (1995). https://doi.org/10.1038/nm0395-267

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