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PCR-mediated recombination: A general method applied to construct chimeric infectious molecular clones of plasma-derived HIV–1 RNA

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Figure 1: Schematic diagram of the PCR–mediated recombination strategy used in constructing HIV–1 chimeric infectious clones.
Figure 2: Agarose gel analysis of DNA fragments amplified by PCR.
Figure 3: Restriction digestion of constructed full–length HIV–1 molecular clones.

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Acknowledgements

We thank E. Shippey and A. Klugo for help in preparing the manuscript and the Wadsworth Center Molecular Genetics Core Laboratory for oligonucleotide synthesis for PCR. This work was supported in part by grants from the National Institutes of Health (RO1AI33334 and U01AI35004 from the National Institute of Allergy and Infectious Diseases and the National Institute on Drug Abuse).

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Correspondence to Harold Burger.

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Fang, G., Weiser, B., Visosky, A. et al. PCR-mediated recombination: A general method applied to construct chimeric infectious molecular clones of plasma-derived HIV–1 RNA. Nat Med 5, 239–242 (1999). https://doi.org/10.1038/5607

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