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Reduction in SIV replication in rhesus macaques infused with autologous lymphocytes engineered with antiviral genes

Nature Medicinevolume 4pages181186 (1998) | Download Citation

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  • An Erratum to this article was published on 01 May 1998

Abstract

Simian immunodeficiency virus (SIV) infection of nonhuman primates is one of the most relevant animals models of HIV infection in humans. To test a potential anti-HIV gene therapy strategy in this model, CD4-enriched lymphocytes from three rhesus macaques were subjected to retrovi-rally mediated gene transfer with a vector expressing an antisense tat/rev gene. This group of animals and three control macaques were subsequently infected with SIVmac239. Blood and lymph nodes from all macaques were sampled for more than a year to monitor the progress of infection. Although all animals became infected, the animals that received the lymphocytes engineered with the antisense vector demonstrated a significant reduction in viral load in both peripheral blood and lymph nodes, had sustained numbers of CD4+ cells, and exhibited little disruption of lymph node architecture.

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Author information

Author notes

    • Bruce A. Bunnell

    Present address: Children's Hospital Research Foundation, 700 Children's Drive, Columbus, Ohio, 43205, USA

Affiliations

  1. Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Room 1B-06, 5 Research Court, Rockville, Maryland, 20901, USA

    • Robert E. Donahue
    • , Mark E. Metzger
    • , Robert P. Westro
    •  & Martha R. Kirby
  2. Clinical Gene Therapy Branch, National Human Genome Research Institute, National Institutes of Health, Building 10, Room IOC103, 10 Center Drive, Bethesda, Maryland, 20892-1851, USA

    • Bruce A. Bunnell
    •  & Richard A. Morgan
  3. Division of Comparative Medicine, Johns Hopkins University School of Medicine, Traylor G-60, 720 Rutland Avenue, Baltimore, Maryland, 21205, USA

    • M. Christine Zink
    • , Tami Unangst
    •  & Janice E. Clements

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DOI

https://doi.org/10.1038/nm0298-181

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