Addendum: Neuronal PTP1B regulates body weight, adiposity and leptin action

Nat. Med. 12, 917–924 (2006); published online 16 July 2006; corrected after print 7 January 2010; addendum published after print 7 January 2010

The widespread use of Nestin-Cre mice has led to an increasing awareness of potential diet- and strain-dependent differences in body weight in these mice. Accordingly, we have tested the effect of Nestin-Cre alone in several strains of mice and have observed marked differences in body weight between Nestin-Cre and wild-type mice fed chow in some, but not all, strains (data not shown). Given these strain differences, and to rule out potential spurious results, we would like to add additional control data from experiments performed at the time of the original studies in our paper showing no effect of Nestin-Cre expression alone on body weight (Fig. 1).

Figure 1: Body weight curves of male mice fed a high-fat diet from weaning (3 weeks of age).

Ptp1b^+/fl Nes-Cre and Ptp1b^fl/fl Nes-Cre mice weighed significantly less than Ptp1b^+/+ Nes-Cre mice by two-way ANOVA with repeated measures (P = 0.013 and P = 0.003, respectively); #P < 0.05 Ptp1b^+/fl Nes-Cre versus Ptp1b^+/+ Nes-Cre, and *P < 0.05 Ptp1b^fl/fl Nes-Cre versus Ptp1b^+/+ Nes-Cre by unpaired two-tailed Student's t test at the indicated time points. Body weights were not different between Ptp1b^+/+ Nes-Cre mice and Ptp1b^+/+ (wild-type), Ptp1b^+/fl or Ptp1b^fl/fl mice fed a high-fat diet from weaning (by two-way ANOVA with repeated measures or two-tailed Student's t test). All mice were on a mixed 129/Sv × C57BL/6 hybrid background.