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Multiplex PCR by multicolor fluorimetry and fluorescence melting curve analysis

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Figure 1: Probe orientation, spectral overlap and color compensation for multicolor Nras mutation detection.
Figure 2: Detection of homozygous point mutations in the Nras gene using a probe complementary to the wild-type Nras sequence.
Figure 3: Fluorescence melting peak analysis of homozygous codon 13 mutants.
Figure 4: Fluorescence melting peak analysis showing heterozygous Nras mutations.


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This work was supported by the ARUP Institute for Clinical and Experimental Pathology and by a Leukemia Research Foundation Grant to KSJE-J and National Institutes of Health Grants (CA83984) to KSJE-J and (GM58983) to CTW.

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Correspondence to Kojo S.J. Elenitoba-Johnson.

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Elenitoba-Johnson, K., Bohling, S., Wittwer, C. et al. Multiplex PCR by multicolor fluorimetry and fluorescence melting curve analysis. Nat Med 7, 249–253 (2001).

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