Abstract

Zika virus (ZIKV) is a flavivirus with teratogenic effects on fetal brain, but the spectrum of ZIKV-induced brain injury is unknown, particularly when ultrasound imaging is normal. In a pregnant pigtail macaque (Macaca nemestrina) model of ZIKV infection, we demonstrate that ZIKV-induced injury to fetal brain is substantial, even in the absence of microcephaly, and may be challenging to detect in a clinical setting. A common and subtle injury pattern was identified, including (i) periventricular T2-hyperintense foci and loss of fetal noncortical brain volume, (ii) injury to the ependymal epithelium with underlying gliosis and (iii) loss of late fetal neuronal progenitor cells in the subventricular zone (temporal cortex) and subgranular zone (dentate gyrus, hippocampus) with dysmorphic granule neuron patterning. Attenuation of fetal neurogenic output demonstrates potentially considerable teratogenic effects of congenital ZIKV infection even without microcephaly. Our findings suggest that all children exposed to ZIKV in utero should receive long-term monitoring for neurocognitive deficits, regardless of head size at birth.

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Acknowledgements

We would like to acknowledge J. Hamanishi for technical assistance with preparation of the figures. We acknowledge V. Alishetti for technical assistance and G. Hess for technical advice related to the flow cytometry studies. We thank M. Diamond (Washington University) for the kind gift of antibodies (ZV-13, Supplementary Table 5). We also thank S. Rodriguez and I. Hansen in the New Mexico State University Biology Department for mosquito rearing. We thank C. Hughes and G. Gallardo for administrative assistance.

This work was primarily supported by generous private philanthropic gifts, mainly from five donors in Florida, who wish to remain anonymous. Further support was obtained from the University of Washington Department of Obstetrics & Gynecology, Seattle Children's Research Institute and the National Institutes of Health (NIH), grant no. R01AI100989 (L.R. and K.M.A.W.), AI083019 (M.G.), AI104002 (M.G.), AI100625 (R.S.B.), AI107731 (R.S.B.), R01NS092339 (R.F.H.), R01NS085081 (R.F.H.), R21OD023838 (B.R.N.), and the Keck Foundation (B.R.N.). The NIH training grants T32 HD007233 (principal investigator: L. Frenkel) and T32 AI07509 (principal investigator: L. Campbell) supported E.B. and J.V., respectively. A Perkins Coie Award for Discovery supported J.A.R. The NIH Office of Research Infrastructure Programs (P51 OD010425) also supported this project. The authors thank the Allen Institute for Brain Science founders, P. Allen and J. Allen, for their vision, encouragement and support.

The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH or other funders. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.

Author information

Author notes

    • Branden R Nelson
    • , Jennifer E Stencel-Baerenwald
    • , Colin Studholme
    • , Raj P Kapur
    • , Blair Armistead
    • , Christie L Walker
    • , Sean Merillat
    • , Jay Vornhagen
    •  & Jennifer Tisoncik-Go

    These authors contributed equally to this work.

Affiliations

  1. Department of Obstetrics & Gynecology, University of Washington, Seattle, Washington, USA.

    • Kristina M Adams Waldorf
    •  & Christie L Walker
  2. Center for Innate Immunity and Immune Disease, University of Washington, Seattle, Washington, USA.

    • Kristina M Adams Waldorf
    • , Jennifer E Stencel-Baerenwald
    • , Jennifer Tisoncik-Go
    • , Justin A Roby
    • , Michael A Davis
    • , Elyse C Dewey
    • , Marian R Fairgrieve
    • , Michael Gale Jr
    •  & Lakshmi Rajagopal
  3. Department of Global Health, University of Washington, Seattle, Washington, USA.

    • Kristina M Adams Waldorf
    • , Blair Armistead
    • , Jay Vornhagen
    • , Michael Gale Jr
    •  & Lakshmi Rajagopal
  4. Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden.

    • Kristina M Adams Waldorf
  5. Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, USA.

    • Branden R Nelson
    • , William B Dobyns
    •  & Robert F Hevner
  6. Department of Immunology, University of Washington, Seattle, Washington, USA.

    • Jennifer E Stencel-Baerenwald
    • , Jennifer Tisoncik-Go
    • , Justin A Roby
    • , Michael A Davis
    • , Elyse C Dewey
    • , Marian R Fairgrieve
    •  & Michael Gale Jr
  7. Department of Pediatrics, University of Washington, Seattle, Washington, USA.

    • Colin Studholme
    • , Michelle Coleman
    • , Erica Boldenow
    • , William B Dobyns
    •  & Lakshmi Rajagopal
  8. Department of Bioengineering, University of Washington, Seattle, Washington, USA.

    • Colin Studholme
    • , Junwei Li
    •  & Xiaohu Gao
  9. Department of Radiology, University of Washington, Seattle, Washington, USA.

    • Colin Studholme
    • , Manjiri K Dighe
    • , Dennis W W Shaw
    • , Jeff Thiel
    •  & Chris Gatenby
  10. Department of Pathology, University of Washington, Seattle, Washington, USA.

    • Raj P Kapur
  11. Department of Pathology, Seattle Children's Hospital, Seattle, Washington, USA.

    • Raj P Kapur
  12. Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.

    • Blair Armistead
    • , Sean Merillat
    • , Jay Vornhagen
    • , Michelle Coleman
    • , Veronica Santana-Ufret
    • , Erica Boldenow
    •  & Lakshmi Rajagopal
  13. Washington National Primate Research Center, Seattle, Washington, USA.

    • Audrey Baldessari
    • , Jason Ogle
    • , G Michael Gough
    • , Wonsok Lee
    • , Chris English
    • , W McIntyre Durning
    • , Richard F Grant
    •  & LaRene Kuller
  14. Department of Radiology, Seattle Children's Hospital, Seattle, Washington, USA.

    • Dennis W W Shaw
  15. Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

    • Jesica A Swanstrom
    • , Kara Jensen
    • , Douglas G Widman
    •  & Ralph S Baric
  16. Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

    • Ralph S Baric
  17. Department of Biology, New Mexico State University, Las Cruces, New Mexico, USA.

    • Joseph T Medwid
    •  & Kathryn A Hanley
  18. Allen Institute for Brain Science, Seattle, Washington, USA.

    • Rebecca D Hodge

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Contributions

K.M.A.W., B.R.N., J.E.S.-B., R.P.K., C.S., R.S.B., D.G.W., M.G. and L.R. designed the study. K.M.A.W., B.R.N., J.E.S.-B., R.P.K., C.S., R.P.K., B.A., S.M., J.T.-G., A.B., M.C., J.A.R., J.V., V.S.-U., E.B., J.A.S., J.L., M.A.D., K.J., D.G.W., J.T.M., K.A.H., J.O., G.M.G., W.L., C.E., W.M.D., C.G., E.C.D., M.R.F., L.K. and L.R. performed the experiments. K.M.A.W., B.R.N., J.E.S.-B., C.S., R.P.K., B.A., C.L.W., S.M., J.T.-G., A.B., M.K.D., D.W.W.S., J.A.R., J.V., J.L., X.G., M.A.D., J.A.S., K.J., R.S.B., R.D.H., R.F.G., W.B.D., R.H., L.R., R.F.H., M.G. and L.R. analyzed the data. K.M.A.W., B.R.N., J.E.S.-B., C.S., R.P.K., B.A., C.L.W., A.B., W.B.D., M.G. and L.R. drafted the manuscript. All authors reviewed the final draft of the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Kristina M Adams Waldorf or Michael Gale Jr or Lakshmi Rajagopal.

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    Supplementary Tables & Figures

    Supplementary Tables 1–5 & Supplementary Figures 1–19

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DOI

https://doi.org/10.1038/nm.4485