Abstract
With the goal of modeling human disease of the large intestine, we sought to develop an effective protocol for deriving colonic organoids (COs) from differentiated human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs). Extensive gene and immunohistochemical profiling confirmed that the derived COs represent colon rather than small intestine, containing stem cells, transit-amplifying cells, and the expected spectrum of differentiated cells, including goblet and endocrine cells. We applied this strategy to iPSCs derived from patients with familial adenomatous polyposis (FAP-iPSCs) harboring germline mutations in the WNT-signaling-pathway-regulator gene encoding APC, and we generated COs that exhibit enhanced WNT activity and increased epithelial cell proliferation, which we used as a platform for drug testing. Two potential compounds, XAV939 and rapamycin, decreased proliferation in FAP-COs, but also affected cell proliferation in wild-type COs, which thus limits their therapeutic application. By contrast, we found that geneticin, a ribosome-binding antibiotic with translational 'read-through' activity, efficiently targeted abnormal WNT activity and restored normal proliferation specifically in APC-mutant FAP-COs. These studies provide an efficient strategy for deriving human COs, which can be used in disease modeling and drug discovery for colorectal disease.
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Gene Expression Omnibus
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Gene Expression Omnibus
Change history
13 March 2018
In the version of this article initially published, there were several instances of duplication of confocal microscopy images presented in Figures 1e, 3i and 4c,h,j. These errors occurred during the final preparation of the figures, when representative images were selected to illustrate the results obtained from multiple independent experiments. To ensure that these errors did not affect the paper’s conclusions, the authors verified all raw data used to quantify the results. The results presented in the original Figures 3j and 4d were not affected, but the quantification in Figure 4i was. This quantification was performed again, and the results supported the original conclusions of the paper. In addition, the western blot shown in Figure 3d came from a different replicate experiment than the one represented in Supplementary Figure 9g,h (which shows the raw data). The original blot has been replaced with the correct blot that matches the raw data originally provided. The conclusions of this experiment remain unchanged. All affected images have been now corrected in the HTML and PDF versions of the article.
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Acknowledgements
S.C. is funded by The New York Stem Cell Foundation (R-103) and NIH/NIDDK (1 DP2 DK098093-01, DP3DK111907-01). This study was supported in part by a shared facility contract from the New York Department of Health (NYSTEM, C029156; to T.E. and S.C.); a Tri-institutional Stem Cell Initiative grant (2013-001; to F. M. and S.C.); and a pilot grant from the Center for Advanced Digestion Care (CADC) at Weill Cornell Medical College (to S.C. and S.L.). S.C. is New York Stem Cell Foundation-Robertson Investigator. This work was also supported by grants R03CA176788 (US National Institutes of Health/National Cancer Institute), the MD Anderson Cancer Center Institutional Research Grant (IRG) Program (E.V.), and a gift from the Feinberg Family to E.V.; Cancer Prevention Educational Award (R25T CA057730, US National Institutes of Health/National Cancer Institute (to K.C.); Arnold O. Beckman postdoctoral fellowship to H.J.C. We thank H.E. Varmus (Weill Cornell Medical College) for his support and J. Jin at Weill Cornell Medical College for kindly providing human aortic smooth muscle cells. We are grateful for the technical support and advice provided by H.S. Ralph in the Cell Screening Core Facility, J. McCormick in the Flow Cytometry Facility and L. Cohen-Gould in the Electron Microscopy Facility at Weill Cornell Medical College.
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S.C., T.E., S.L., and F.R.M. designed the project; M.C., S.-Y.T., S.A., T.S., N.H.P.H.J.C., M.W., and M.G. performed experiments; E.V., K.C., T. Z., and J.Z.X. performed the bioinformatics analysis; M.C., E.V., K.C., and S.C. analyzed data; and M.C., E.V., F.R.M., S.L., T.E., and S.C. wrote the manuscript.
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Crespo, M., Vilar, E., Tsai, SY. et al. Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing. Nat Med 23, 878–884 (2017). https://doi.org/10.1038/nm.4355
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DOI: https://doi.org/10.1038/nm.4355
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