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MAFB prevents excess inflammation after ischemic stroke by accelerating clearance of damage signals through MSR1

Nature Medicine volume 23, pages 723–732 (2017)Cite this article

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Abstract

Damage-associated molecular patterns (DAMPs) trigger sterile inflammation after tissue injury, but the mechanisms underlying the resolution of inflammation remain unclear. In this study, we demonstrate that common DAMPs, such as high-mobility-group box 1 (HMGB1), peroxiredoxins (PRXs), and S100A8 and S100A9, were internalized through the class A scavenger receptors MSR1 and MARCO in vitro. In ischemic murine brain, DAMP internalization was largely mediated by MSR1. An elevation of MSR1 levels in infiltrating myeloid cells observed 3 d after experimental stroke was dependent on the transcription factor Mafb. Combined deficiency for Msr1 and Marco, or for Mafb alone, in infiltrating myeloid cells caused impaired clearance of DAMPs, more severe inflammation, and exacerbated neuronal injury in a murine model of ischemic stroke. The retinoic acid receptor (RAR) agonist Am80 increased the expression of Mafb, thereby enhancing MSR1 expression. Am80 exhibited therapeutic efficacy when administered, even at 24 h after the onset of experimental stroke. Our findings uncover cellular mechanisms contributing to DAMP clearance in resolution of the sterile inflammation triggered by tissue injury.

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Figure 1: PRXs, HMGB1, and S100A8/A9 are internalized by infiltrating mononuclear phagocytes.
Figure 2: Identification of Msr1 and Mafb as essential genes for the internalization of DAMPs.
Figure 3: Mafb-dependent enhanced MSR1 expression and characteristics of the MSR1hi myeloid fraction in ischemic brain.
Figure 4: Impaired clearance of DAMPs due to Msr1/Marco deficiency exacerbates the pathology of ischemic stroke.
Figure 5: The dominant roles of MSR1–MARCO and MAFB in infiltrating myeloid cells for the pathologies of ischemic stroke.
Figure 6: Therapeutic effect of Am80 through its promotion of MAFB-dependent enhanced MSR1 expression.

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Acknowledgements

We thank N. Shiino, A. Ino, and M. Asakawa for their technical assistance and H. Yao for his technical advice on the MCAO model. Msr1/Marco-deficient mice were kindly provided by K. Tryggvason (Duke–NUS Medical School). This work was supported by PRESTO from the Japan Science and Technology Agency (T.S.), a Grant-in-Aid for Scientific Research on Innovative Areas (Homeostatic regulation by various types of cell death) (15H01387) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) (T.S.), JSPS KAKENHI Grants-in-Aid for Young Scientists (B) (26870571) (T.S.) and (S) (25221305) (A.Y.), Advanced Research & Development Programs for Medical Innovation (AMED-CREST) (A.Y.), a Toray Science and Technology Grant (T.S.), the Takeda Science Foundation (T.S.), the Mochida Memorial Foundation for Medical and Pharmaceutical Research (T.S.), a Japan Heart Foundation Research Grant (T.S.), the SENSHIN Medical Research Foundation (A.Y.), Keio Gijuku Academic Developmental Funds (A.Y.), and Open Research for Young Academics and Specialists from MEXT (A.Y.).

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Authors and Affiliations

  1. Department of Microbiology and Immunology, School of Medicine, Keio University, Tokyo, Japan

    Takashi Shichita, Minako Ito, Rimpei Morita, Kyoko Komai, Yoshiko Noguchi & Akihiko Yoshimura

  2. Core Research for Evolutional Science and Technology (CREST), Japan Agency for Medical Research and Development (AMED), Tokyo, Japan

    Takashi Shichita, Minako Ito, Rimpei Morita, Kyoko Komai, Yoshiko Noguchi & Akihiko Yoshimura

  3. Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Tokyo, Japan

    Takashi Shichita

  4. Stroke Renaissance Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

    Takashi Shichita

  5. Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

    Takashi Shichita

  6. Department of Internal Medicine, Fukuoka Dental College Medical and Dental Hospital, Fukuoka, Japan

    Hiroaki Ooboshi

  7. Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan

    Ryusuke Koshida & Satoru Takahashi

  8. Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, Japan

    Tatsuhiko Kodama

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Contributions

T.S. designed and performed the experiments, analyzed the data, and wrote the manuscript; Y.N., M.I., and K.K. performed the experiments; R.M. and H.O. provided technical advice; R.K. and S.T. provided Lysm-Cre; Mafbflox/flox mice; T.K. provided Msr1/Marco double-knockout mice; and A.Y. directed the entire study, designed the experiments, and wrote the manuscript.

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Correspondence to Takashi Shichita or Akihiko Yoshimura.

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Shichita, T., Ito, M., Morita, R. et al. MAFB prevents excess inflammation after ischemic stroke by accelerating clearance of damage signals through MSR1. Nat Med 23, 723–732 (2017). https://doi.org/10.1038/nm.4312

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