Brief Communication | Published:

Diagnosis of Zika virus infection on a nanotechnology platform

Nature Medicine volume 23, pages 548550 (2017) | Download Citation

Abstract

We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections.

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Author information

Author notes

    • Bo Zhang
    •  & Benjamin A Pinsky

    These authors contributed equally to this work.

Affiliations

  1. Nirmidas Biotech, Palo Alto, California, USA.

    • Bo Zhang
    • , Jeyarama S Ananta
    • , Su Zhao
    • , Shylaja Arulkumar
    • , Clay Hopes
    •  & Meijie Tang
  2. Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.

    • Benjamin A Pinsky
    •  & Malaya K Sahoo
  3. Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA.

    • Benjamin A Pinsky
  4. Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen, China.

    • Hao Wan
  5. Medical Research Institute, Colombo, Sri Lanka.

    • Janaki Abeynayake
  6. Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.

    • Jesse J Waggoner
  7. Department of Chemistry, Stanford University, Stanford, California, USA.

    • Hongjie Dai

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Contributions

B.Z., M.T. and H.D. conceived of the study. B.Z., B.A.P. and H.D. designed the experiments. J.A. and B.A.P. provided serum samples. B.Z., J.S.A., S.A., S.Z., H.W., M.K.S. and C.H. conducted the experiments. B.Z., J.J.W., B.A.P. and H.D. analyzed the data and wrote the manuscript.

Competing interests

H. Dai is a scientific adviser and share-holder of Nirmidas Biotech and served as a consultant for this work. The pGOLD platform was licensed to Nirmidas Biotech by Stanford University.

Corresponding authors

Correspondence to Bo Zhang or Hongjie Dai.

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    Supplementary Figures and Tables

    Supplementary Figures 1–7 and Supplementary Tables 1–5

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DOI

https://doi.org/10.1038/nm.4302

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