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Diagnosis of Zika virus infection on a nanotechnology platform

Nature Medicine volume 23, pages 548–550 (2017)Cite this article

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Abstract

We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections.

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Figure 1: ZIKV and DENV IgG, IgM, IgA and IgG avidity levels, as measured using the pGOLD platform, for three groups of individuals: a cohort of 29 patients with ZIKV infection (Group1_Z), a cohort of 64 patients with DENV infection (Group2_D) and a control group of 50 samples (Group3_H).
Figure 2: Serology of acute ZIKV infection in patients positive for ZIKV RNA in serum (Group4_Z) and acute secondary DENV infection in patients positive for DENV RNA in serum (Group5_DD).

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Author information

Author notes

  1. Bo Zhang and Benjamin A Pinsky: These authors contributed equally to this work.

Authors and Affiliations

  1. Nirmidas Biotech, Palo Alto, California, USA

    Bo Zhang, Jeyarama S Ananta, Su Zhao, Shylaja Arulkumar, Clay Hopes & Meijie Tang

  2. Department of Pathology, Stanford University School of Medicine, Stanford, California, USA

    Benjamin A Pinsky & Malaya K Sahoo

  3. Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA

    Benjamin A Pinsky

  4. Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen, China

    Hao Wan

  5. Medical Research Institute, Colombo, Sri Lanka

    Janaki Abeynayake

  6. Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA

    Jesse J Waggoner

  7. Department of Chemistry, Stanford University, Stanford, California, USA

    Hongjie Dai

Authors
  1. Bo Zhang
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  2. Benjamin A Pinsky
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  3. Jeyarama S Ananta
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  4. Su Zhao
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  5. Shylaja Arulkumar
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  6. Hao Wan
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  7. Malaya K Sahoo
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  8. Janaki Abeynayake
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  9. Jesse J Waggoner
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  10. Clay Hopes
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  11. Meijie Tang
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  12. Hongjie Dai
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Contributions

B.Z., M.T. and H.D. conceived of the study. B.Z., B.A.P. and H.D. designed the experiments. J.A. and B.A.P. provided serum samples. B.Z., J.S.A., S.A., S.Z., H.W., M.K.S. and C.H. conducted the experiments. B.Z., J.J.W., B.A.P. and H.D. analyzed the data and wrote the manuscript.

Corresponding authors

Correspondence to Bo Zhang or Hongjie Dai.

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Competing interests

H. Dai is a scientific adviser and share-holder of Nirmidas Biotech and served as a consultant for this work. The pGOLD platform was licensed to Nirmidas Biotech by Stanford University.

Supplementary information

Supplementary Figures and Tables

Supplementary Figures 1–7 and Supplementary Tables 1–5 (PDF 1443 kb)

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Zhang, B., Pinsky, B., Ananta, J. et al. Diagnosis of Zika virus infection on a nanotechnology platform. Nat Med 23, 548–550 (2017). https://doi.org/10.1038/nm.4302

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