Figure 2 : Strategies for the generation, characterization and systems-level analysis of 3D cellular models of the human brain.

From: The promises and challenges of human brain organoids as models of neuropsychiatric disease

Figure 2

Banks of disease-relevant hPSC lines can be established from patient cohorts or through genetic engineering to introduce specific risk variants. 3D brain organoids developed from these lines can be used for systems-level analyses of cellular, circuit and architectural properties; methods for data integration across these modalities will need to be established. The fidelity with which 3D organoids and spheroids replicate the human brain must also be established. Next-generation organoid protocols will aim to produce advanced neural-network maturation, greater integration of diverse cell types and increased reproducibility to enable analysis of additional processes relevant to neuropsychiatric disorders.