Experimental modeling of cancer typically uses in vitro culture of transformed cell lines or in vivo animal models. A new study using CRISPR-Cas9 to engineer oncogenic mutations into three-dimensional human colon organoid cultures yields insights into colorectal cancer tumorigenesis.
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C.J.K. is an inventor on a pending patent describing organoid culture methodologies.
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Salahudeen, A., Kuo, C. Toward recreating colon cancer in human organoids. Nat Med 21, 215–216 (2015). https://doi.org/10.1038/nm.3818
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DOI: https://doi.org/10.1038/nm.3818
