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Developmental biology using purified genes

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Figure 1: Comparison of control (left), anucleolate mutant (center) and magnesium-deficient (right) embryos of X. laevis after four days of development.
Figure 2: An isolated nucleus from a mature oocyte of X. laevis.
Figure 3: Electron micrograph of a partially denatured molecule with repeating units of 5S DNA.
Figure 4: A transcriptional-control region within the 5S rRNA gene.


  1. Elsdale, T.R., Fischberg, M. & Smith, S. A mutation that reduces nucleolar number in Xenopus laevis. Exp. Cell Res. 14, 642–643 (1958).

    Article  CAS  Google Scholar 

  2. Brown, D.D. & Gurdon, J.B. Absence of ribosomal RNA synthesis in the anucleolate mutant of Xenopus laevis. Proc. Natl. Acad. Sci. USA 51, 139–146 (1964).

    Article  CAS  Google Scholar 

  3. Wallace, H. & Birnstiel, M. Ribosomal cistrons and the nucleolar organizer. Biochim. Biophys. Acta 114, 296–310 (1966).

    Article  CAS  Google Scholar 

  4. Miller, O.L. & Beatty, B.R. Visualization of nucleolar genes. Science 164, 955–957 (1969).

    Article  Google Scholar 

  5. Brown, D.D. & Dawid, I.B. Specific gene amplification in oocytes. Science 160, 272–280 (1968).

    Article  CAS  Google Scholar 

  6. Gall, J.G. Differential synthesis of the genes for ribosomal RNA during amphibian oogenesis. Proc. Natl. Acad. Sci. USA 60, 553–560 (1968).

    Article  CAS  Google Scholar 

  7. Brown, D.D., Wensink, P.C. & Jordan, E. Purification and some characteristics of 5S DNA from Xenopus laevis. Proc. Natl. Acad. Sci. USA 68, 3175–3179 (1971).

    Article  CAS  Google Scholar 

  8. Miller, J.R. et al. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region. Cell 13, 717–725 (1978).

    Article  CAS  Google Scholar 

  9. Brown, D.D. The role of stable complexes that repress and activate eucaryotic genes. Cell 37, 359–365 (1984).

    Article  CAS  Google Scholar 

  10. Birkenmeier, E.H., Brown, D.D. & Jordan, E. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes. Cell 15, 1077–1086 (1978).

    Article  CAS  Google Scholar 

  11. Sakonju, S., Bogenhagen, D.F. & Brown, D.D. A control region in the center of the 5S RNA gene directs specific initiation of transcription. I. The 5′ border of the region. Cell 19, 13–25 (1980).

    Article  CAS  Google Scholar 

  12. Bogenhagen, D.F., Sakonju, S. & Brown, D.D. A control region in the center of the 5S RNA gene directs specific initiation of transcription. II. The 3′ border of the region. Cell 19, 27–35 (1980).

    Article  CAS  Google Scholar 

  13. Engelke, D.R. et al. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. Cell 19, 717–728 (1980).

    Article  CAS  Google Scholar 

  14. Sakonju, S. et al. The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene. Cell 23, 665–669 (1981).

    Article  CAS  Google Scholar 

  15. Miller, J., McLachlan, A. & Klug, A. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J. 4, 1609–1614 (1985).

    Article  CAS  Google Scholar 

  16. Suzuki, Y. & Brown, D.D. Isolation and identification of the messenger RNA for the silk fibroin from Bombyx mori. J. Mol. Biol. 63, 409–429 (1972).

    Article  CAS  Google Scholar 

  17. Brown, D.D. & Cai, L. Amphibian metamorphosis. Dev. Biol. 306, 20–33 (2007).

    Article  CAS  Google Scholar 

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I owe a great deal to the Carnegie Institution of Washington, now called the Carnegie Institute for Science. It has supported my research for 50 years. I have had generous grants from the US National Institute of General Medical Science since 1975. The G. Harold & Leila Y. Mathers Foundation supported my research in amphibian metamorphosis. Many wonderful graduate students, postdoctoral fellows and other colleagues have contributed to these studies. It is a special pleasure to share the responsibility of running the LSRF with D. Koshland. He and I are responsible for finding sponsors who will support postdoctoral fellows. C. Pratt carries out the day-to-day decisions with the highest efficiency and intelligence. S. DiRenzo, T. Silhavy and J. Broach administer LSRF's peer review process at Princeton University, which this year reached its highest application number ever of 900.

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Correspondence to Donald D Brown.

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Brown, D. Developmental biology using purified genes. Nat Med 18, 1496–1498 (2012).

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