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Technical concerns about immunoprecipitation of methylated fetal DNA for noninvasive trisomy 21 diagnosis

Nature Medicine volume 18, pages 1327–1328 (2012)Cite this article

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To the Editor:

We read with great interest the paper by Papageorgiou et al.1 on the use of fetal-specific DNA methylation ratio for the noninvasive prenatal diagnosis of trisomy 21. The authors focused on a number of differentially methylated regions that showed different methylation patterns between placental and maternal blood DNA2. They used methylated DNA immunoprecipitation (MeDIP) followed by real-time quantitative PCR (qPCR) on DNA extracted from maternal blood for the detection of fetal trisomy 21.

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Figure 1: D values in euploid pregnancies, trisomy 21 pregnancies and technical replicates.

References

  1. Papageorgiou, E.A. et al. Nat. Med. 17, 510–513 (2011).

    Article  CAS  Google Scholar 

  2. Papageorgiou, E.A. et al. Am. J. Pathol. 174, 1609–1618 (2009).

    Article  CAS  Google Scholar 

  3. Bianchi, D.W. et al. Am. J. Hum. Genet. 61, 822–829 (1997).

    Article  CAS  Google Scholar 

  4. Lo, Y.M.D. et al. Am. J. Hum. Genet. 62, 768–775 (1998).

    Article  CAS  Google Scholar 

  5. Tong, Y.K. et al. Clin. Chem. 56, 90–98 (2010).

    Article  CAS  Google Scholar 

  6. Lun, F.M.F. et al. Clin. Chem. 54, 1664–1672 (2008).

    Article  CAS  Google Scholar 

  7. Hamada, H., Arinami, T., Kubo, T., Hamaguchi, H. & Iwasaki, H. Hum. Genet. 91, 427–432 (1993).

    Article  CAS  Google Scholar 

  8. Bianchi, D.W. et al. Prenat. Diagn. 22, 609–615 (2002).

    Article  CAS  Google Scholar 

  9. Zweig, M.H. & Campbell, G. Clin. Chem. 39, 561–577 (1993).

    CAS  Google Scholar 

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Acknowledgements

This project was supported by the University Grants Committee of the Government of the Hong Kong Special Administration Region, China—Areas of Excellence Scheme (AoE/M-04/06). We thank A. Cheng, S. Lau, L. Lu and P. Jiang for helpful discussions.

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Authors and Affiliations

  1. Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR, China

    Yu Kwan Tong, Rossa Wai Kwun Chiu, Kwan Chee Allen Chan & Yuk Ming Dennis Lo

  2. Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR, China

    Yu Kwan Tong, Rossa Wai Kwun Chiu, Kwan Chee Allen Chan & Yuk Ming Dennis Lo

  3. Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR, China

    Tak Yeung Leung

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  1. Yu Kwan Tong
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Correspondence to Yuk Ming Dennis Lo.

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Competing interests

All authors hold or have filed patent applications on aspects of noninvasive prenatal diagnosis. Part of this patent portfolio has been licensed to Sequenom. Y.M.D.L. and R.W.K.C. are consultants to, hold equities in, and receive research support from Sequenom.

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Supplementary Tables 1–4, Supplementary Figure 1 and Supplementary Methods (PDF 294 kb)

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Tong, Y., Chiu, R., Chan, K. et al. Technical concerns about immunoprecipitation of methylated fetal DNA for noninvasive trisomy 21 diagnosis. Nat Med 18, 1327–1328 (2012). https://doi.org/10.1038/nm.2915

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