Abstract
Atherosclerosis and its consequences remain the main cause of mortality in industrialized and developing nations. Plaque burden and progression have been shown to be independent predictors for future cardiac events by intravascular ultrasound. Routine prospective imaging is hampered by the invasive nature of intravascular ultrasound. A noninvasive technique would therefore be more suitable for screening of atherosclerosis in large populations. Here we introduce an elastin-specific magnetic resonance contrast agent (ESMA) for noninvasive quantification of plaque burden in a mouse model of atherosclerosis. The strong signal provided by ESMA allows for imaging with high spatial resolution, resulting in accurate assessment of plaque burden. Additionally, plaque characterization by quantifying intraplaque elastin content using signal intensity measurements is possible. Changes in elastin content and the high abundance of elastin during plaque development, in combination with the imaging properties of ESMA, provide potential for noninvasive assessment of plaque burden by molecular magnetic resonance imaging (MRI).
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Acknowledgements
This study was funded by a BHF project grant (PG/09/061) awarded to R.M.B. M.R.M. was partly funded by a BHF studentship awarded to R.M.B. ESMA (BMS753951) was provided by Lantheus Medical Imaging. Supporting data for the in vitro rabbit competition and in vivo mouse distribution studies were provided by P. Yalamanchili, M. Kavosi and P. Silva from Lantheus Medical Imaging.
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M.R.M. and R.M.B. are responsible for the overall study design and implemented and optimized the magnetic resonance imaging protocols. D.C.O., R.R.C. and S.P.R. designed and manufactured the contrast agent. U.B. and T.S. developed and implemented the T1 mapping sequence and analysis tools. M.R.M., R.M.B., A.J.W., A.S. and F.C. designed, conducted and analyzed the in vitro and in vivo experiments. A.W. performed the electron microscopy experiments. M.R.M., R.M.B., A.J.W., F.C., M.S.M., E.N., T.S., A.S., R.R. and C.H.P.J. contributed to the writing of the manuscript. All authors discussed and refined the manuscript.
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The magnetic resonance imaging scanner is partly supported by Philips Healthcare. A.J.W.is an employee of Philips Healthcare. D.C.O., R.R.C. and S.P.R. are employees of Lantheus Medical Imaging. The study was funded by the British Heart Foundation (PG/09/061), and the contrast agent was provided by Lantheus Medical Imaging.
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Supplementary Figures 1–3 and Supplementary Methods (PDF 2378 kb)
Supplementary Video 1
3D reconstruction (volume rendering) of elastin signal in the brachiocephalic artery of an Apoe−/− mouse. (MOV 257 kb)
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Makowski, M., Wiethoff, A., Blume, U. et al. Assessment of atherosclerotic plaque burden with an elastin-specific magnetic resonance contrast agent. Nat Med 17, 383–388 (2011). https://doi.org/10.1038/nm.2310
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DOI: https://doi.org/10.1038/nm.2310
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