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Validated germline-competent embryonic stem cell lines from nonobese diabetic mice


Nonobese diabetic (NOD) mice provide an excellent model of type 1 diabetes. The genetic contribution to this disease is complex, with more than 20 loci implicated in diabetes onset. One of the challenges for researchers using the NOD mouse model (and, indeed, other models of spontaneous autoimmune disease) has been the high density of sequence variation within candidate chromosomal segments. Furthermore, the scope for analyzing many putative disease loci via gene targeting has been hampered by the lack of NOD embryonic stem (ES) cells. We describe here the derivation of NOD ES cell lines capable of generating chimeric mice after stable genetic modification. These NOD ES cell lines also show efficient germline transmission, with offspring developing diabetes. The availability of these cells will not only enable the dissection of closely linked loci and the role they have in the onset of type 1 diabetes but also facilitate the generation of new transgenics.

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Figure 1: Characterization of NOD ES cells.
Figure 2: NOD ES cells generate chimeric mice.
Figure 3: NOD ES cells support efficient germline transmission.
Figure 4: DsRED expression in chimeric mice and F1 germline progeny.

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We would like to thank G. Guo (Wellcome Trust Centre for Stem Cell Research) for providing the PiggyBac DsRED vector used in our studies, R. Zamoyska (University of Edinburgh) for the FITC-labeled antibody to H-2Kb and K. Tomonari (Fukui Medical School) for the KT3 antibody. We thank N. Holmes and J. Cooke for careful reading of our manuscript. We thank C. Bland and Y. Sawyer for technical assistance. We are grateful to the Wellcome Trust and the Medical Research Council for supporting this research.

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J.N. contributed to the design of the experiments, supervised and conducted the experiments and was involved in writing the manuscript;, K.J. helped conduct the experiments and generated the genetically modified NOD ES cells; J.M.P. helped conduct experiments, conducted all of the in vivo and ex vivo NOD work and generated the manuscript figures; S.A.N. conducted all of the microsatellite experiments in Figures 2 and 3; M.R. helped in the conduct of experiments in Figure 1; W.M. performed the blastocyst injections; A.S. contributed to the design of the experiments and the review of the manuscript; and A.C. contributed to the design of the experiments and supervised and was involved in writing the manuscript.

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Correspondence to Anne Cooke.

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Nichols, J., Jones, K., Phillips, J. et al. Validated germline-competent embryonic stem cell lines from nonobese diabetic mice. Nat Med 15, 814–818 (2009).

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