Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Adenovirus-specific immunity after immunization with an Ad5 HIV-1 vaccine candidate in humans

Nature Medicine volume 15, pages 873–875 (2009)Cite this article

Abstract

The immunologic basis for the potential enhanced HIV-1 acquisition in adenovirus serotype 5 (Ad5)-seropositive individuals who received the Merck recombinant Ad5 HIV-1 vaccine in the STEP study remains unclear. Here we show that baseline Ad5-specific neutralizing antibodies are not correlated with Ad5-specific T lymphocyte responses and that Ad5-seropositive subjects do not develop higher vector-specific cellular immune responses as compared with Ad5-seronegative subjects after vaccination. These findings challenge the hypothesis that activated Ad5-specific T lymphocytes were the cause of the potential enhanced HIV-1 susceptibility in the STEP study.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Get just this article for as long as you need it

$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Adenovirus-specific humoral and cellular immune responses before and after rAd5-Gag vaccination.
Figure 2: Magnitude and phenotype of adenovirus-specific and total CD4+ T lymphocyte subpopulations before and after 1 × 1011 vp rAd5-Gag vaccination.

References

  1. Buchbinder, S.P. et al. Lancet 372, 1881–1893 (2008).

    Article  CAS  Google Scholar 

  2. McElrath, M.J. et al. Lancet 372, 1894–1905 (2008).

    Article  CAS  Google Scholar 

  3. Priddy, F.H. et al. Clin. Infect. Dis. 46, 1769–1781 (2008).

    Article  CAS  Google Scholar 

  4. Sprangers, M.C. et al. J. Clin. Microbiol. 41, 5046–5052 (2003).

    Article  CAS  Google Scholar 

  5. Liu, J. et al. Nature 457, 87–91 (2009).

    Article  CAS  Google Scholar 

  6. Vogels, R. et al. J. Virol. 77, 8263–8271 (2003).

    Article  CAS  Google Scholar 

  7. Abbink, P. et al. J. Virol. 81, 4654–4663 (2007).

    Article  CAS  Google Scholar 

  8. Barouch, D.H. et al. J. Immunol. 172, 6290–6297 (2004).

    Article  CAS  Google Scholar 

  9. Catanzaro, A.T. et al. J. Infect. Dis. 194, 1638–1649 (2006).

    Article  CAS  Google Scholar 

  10. Thorner, A.R. et al. J. Clin. Microbiol. 44, 3781–3783 (2006).

    Article  Google Scholar 

  11. Liu, J. et al. J. Virol. 82, 4844–4852 (2008).

    Article  CAS  Google Scholar 

  12. Perreau, M., Pantaleo, G. & Kremer, E.J. J. Exp. Med. 205, 2717–2725 (2008).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank J. Custers, M. Pau, P. Abbink, A. La Porte, N. Letvin, F. Stephens and T. Swanson for generous advice and assistance. We acknowledge support from the Bill & Melinda Gates Foundation Collaboration for AIDS Vaccine Discovery (38614) and the US National Institutes of Health (AI066305, AI066924, AI078526, AI074078 and AI076066).

Author information

Authors and Affiliations

  1. Division of Vaccine Research, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA

    Kara L O'Brien, Jinyan Liu, Sharon L King, Ying-Hua Sun & Dan H Barouch

  2. Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA

    Joern E Schmitz & Michelle A Lifton

  3. Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA

    Natalie A Hutnick & Michael R Betts

  4. Merck Research Laboratories, West Point, Pennsylvania, USA

    Sheri A Dubey, John W Shiver, Michael N Robertson & Danilo R Casimiro

  5. Crucell Holland BV, Leiden, The Netherlands

    Jaap Goudsmit

  6. Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, USA

    Dan H Barouch

Authors
  1. Kara L O'Brien
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jinyan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sharon L King
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ying-Hua Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Joern E Schmitz
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Michelle A Lifton
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Natalie A Hutnick
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Michael R Betts
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Sheri A Dubey
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jaap Goudsmit
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. John W Shiver
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Michael N Robertson
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Danilo R Casimiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Dan H Barouch
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

K.L.O. and J.L. performed and analyzed the cellular immunologic assays. S.L.K. and Y.-H.S. performed and analyzed the humoral immunologic assays. J.E.S., M.A.L., N.A.H. and M.R.B. provided reagents and performed flow cytometric assays. S.A.D., J.W.S., M.N.R. and D.R.C. provided clinical samples and participated in the design of the study. J.G. provided vectors and participated in the design of the study. D.H.B. led the design and conduct of the study. All coauthors discussed the data and contributed to writing the manuscript.

Corresponding author

Correspondence to Dan H Barouch.

Supplementary information

Supplementary Text and Figures

Supplementary Figs. 1–4 and Supplementary Methods (PDF 486 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

O'Brien, K., Liu, J., King, S. et al. Adenovirus-specific immunity after immunization with an Ad5 HIV-1 vaccine candidate in humans. Nat Med 15, 873–875 (2009). https://doi.org/10.1038/nm.1991

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nm.1991

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing