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Decreased levels of microRNA miR-122 in individuals with hepatitis C responding poorly to interferon therapy

Abstract

Several microRNAs (miRNAs), including liver-specific miR-122, have been implicated in the control of hepatitis C virus (HCV) RNA replication and its response to interferon (IFN) in human hepatoma cells. Our analysis of liver biopsies from subjects with chronic hepatitis C (CHC) undergoing IFN therapy revealed no correlation of miR-122 expression with viral load and markedly decreased pretreatment miR-122 levels in subjects who had no virological response during later IFN therapy; other investigated miRNAs showed only limited changes. These data have implications for the prospect of targeting miRNAs for CHC therapy.

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Figure 1: MiR-122 levels in livers of subjects with CHC.
Figure 2: Effect of IFN on levels of miRNAs in mouse liver and human hepatoma Huh7 cells.

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References

  1. Jopling, C.L., Yi, M., Lancaster, A.M., Lemon, S.M. & Sarnow, P. Science 309, 1577–1581 (2005).

    Article  CAS  Google Scholar 

  2. Randall, G. et al. Proc. Natl. Acad. Sci. USA 104, 12884–12889 (2007).

    Article  CAS  Google Scholar 

  3. Manns, M.P. et al. Lancet 358, 958–965 (2001).

    Article  CAS  Google Scholar 

  4. Fried, M.W. et al. N. Engl. J. Med. 347, 975–982 (2002).

    Article  CAS  Google Scholar 

  5. Pedersen, I.M. et al. Nature 449, 919–922 (2007).

    Article  CAS  Google Scholar 

  6. Sarasin-Filipowicz, M. et al. Proc. Natl. Acad. Sci. USA 105, 7034–7039 (2008).

    Article  CAS  Google Scholar 

  7. Brown, B.D. et al. Nat. Biotechnol. 25, 1457–1467 (2007).

    Article  CAS  Google Scholar 

  8. Griffiths-Jones, S., Saini, H.K., van Dongen, S. & Enright, A.J. Nucleic Acids Res. 36, D154–D158 (2008).

    Article  CAS  Google Scholar 

  9. Elmen, J. et al. Nature 452, 896–899 (2008).

    Article  CAS  Google Scholar 

  10. Chen, L. et al. Gastroenterology 128, 1437–1444 (2005).

    Article  CAS  Google Scholar 

  11. Asselah, T. et al. Gut 57, 516–524 (2008).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to thank the subjects who participated in this study. The work done in the laboratory of M.H.H. is supported by Swiss National Science Foundation grant 32-116106, the Swiss Cancer League grant KLS-01832-02-2006, grant 8/05 from the Krebsliga Basel and a grant from the Roche Research Foundation to M.S.-F. The work done in the laboratory of W.F. is supported partially by the EC FP6 Program “Sirocco”. J.K. is the recipient of a Long-Term European Molecular Biology Organization Fellowship, and the Friedrich Miescher Institute is supported by the Novartis Research Foundation.

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Contributions

M.S.-F., J.K., M.H.H. and W.F. conceived and designed the experiments; M.S.-F., J.K. and I.M. performed the experiments; M.S.-F. and J.K. analyzed the data and contributed to writing and editing the manuscript; M.H.H. and W.F. supervised the project and wrote the manuscript.

Corresponding authors

Correspondence to Markus H Heim or Witold Filipowicz.

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Supplementary Methods, Supplementary Table 1 and Supplementary Figs. 1–8 (PDF 621 kb)

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Sarasin-Filipowicz, M., Krol, J., Markiewicz, I. et al. Decreased levels of microRNA miR-122 in individuals with hepatitis C responding poorly to interferon therapy. Nat Med 15, 31–33 (2009). https://doi.org/10.1038/nm.1902

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