Mast cell–dependent migration of effector CD8+ T cells through production of leukotriene B4

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Studies in both humans and rodents indicate that CD8+ T cells may be important in allergic inflammation. However, neither the mechanisms that mediate CD8+ T cell recruitment to inflamed tissues nor the relative participation of effector and central memory CD8+ T cells is known. Here we report that activated mast cells induced chemotaxis of effector, but not central memory, CD8+ T cells through production of leukotriene B4 (LTB4). These studies indicate that LTB4 production by activated peripheral leukocytes could be important for the recruitment of effector CD8+ T cells to sites of inflammation.

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Figure 1: Activated mast cells induce selective migration of TEFF cells.
Figure 2: Mast cell–induced TEFF cell migration is dependent on mast cell production of leukotrienes.
Figure 3: LTB4 induces migration of TEFF cells, but not TCM cells.
Figure 4: LTB4-induced TEFF cell migration is mediated by BLT1.
Figure 5: LTB4-induced TEFF cell migration requires a PTX-sensitive pathway.
Figure 6: Activated CD8+ T cells do not migrate to LTB4.
Figure 7: LTB4 sensitivity is induced by TCR aggregation and culture in IL-2.

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The authors thank R.C. Murphy for contributions to this work, and B. Townend, S. Sobus and J. Loomis for technical assistance with cell sorting. This work was supported by US Public Health Service grants AI-17134, AI-18785, AI-52225 and AI-22295 (to P.M. and J.K.), and AI-39773 (to J.C.C.). V.L.O. is supported by a National Research Service Award from the National Institutes of Health. B.J.S. is supported by a Norman B. Levy Fellowship in Basic Immunological Research from National Jewish Medical and Research Center.

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Correspondence to Vanessa L Ott.

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