The elimination of viruses and tumors by natural killer cells is mediated by specific natural killer cell receptors. To study the in vivo function of a principal activating natural killer cell receptor, NCR1 (NKp46 in humans), we replaced the gene encoding this receptor (Ncr1) with a green fluorescent protein reporter cassette. There was enhanced spread of certain tumors in 129/Sv but not C57BL/6 Ncr1gfp/gfp mice, and influenza virus infection was lethal in both 129/Sv and C57BL/6 Ncr1gfp/gfp mice. We noted accumulation of natural killer cells at the site of influenza infection by tracking the green fluorescent protein. Our results demonstrate a critical function for Ncr1 in the in vivo eradication of influenza virus.
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Supported by the Israel Science Foundation (O.M.), the Binational Science Foundation (O.M.), the Israel Ministry of Health and the European Commission (LSHC-CT-2002-518178 to O.M.).
Generation of Ncr1gfp/gfp mice. (PDF 541 kb)
Ncr1gfp expression in NK cells after PGK-neor excision in 129/sv mice. (PDF 402 kb)
NK cell-specific Ncr1gfp expression in 129/sv mice after PGK-neor excision. (PDF 401 kb)
C57BL/6 mice express Ncr1gfp in NK cells. (PDF 426 kb)
NK cell-specific Ncr1gfp expression in C57BL/6 mice. (PDF 421 kb)
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