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Fcα/μ receptor mediates endocytosis of IgM-coated microbes

Nature Immunology volume 1, pages 441–446 (2000)Cite this article

Abstract

IgM is the first antibody to be produced in a humoral immune response and plays an important role in the primary stages of immunity. Here we describe a mouse Fc receptor, designated Fcα/μR, and its human homolog, that bind both IgM and IgA with intermediate or high affinity. Fcα/μR is constitutively expressed on the majority of B lymphocytes and macrophages. Cross-linking Fcα/μR expressed on a pro-B cell line Ba/F3 transfectant with soluble IgM or IgM-coated microparticles induced internalization of the receptor. Fcα/μR also mediated primary B lymphocyte endocytosis of IgM-coated Staphylococcus aureus. Thus, Fcα/μR is involved in the primary stages of the immune response to microbes.

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Figure 1: Molecular and biochemical characterization of Fcα/μR.
Figure 2: Expression of mFcα/μR.
Figure 3: Binding of immunoglobulins to mFcα/μR.
Figure 4: Internalization of mFcα/μR.

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Acknowledgements

We thank A. Iwama, Y. Nakamura, H. Ema, M. Onodera, H. Miyoshi for discussions; Y. Morita and R. Shimamura for assistance with flow cytometry; and M. Ito for secretarial assistance. Supported in part by grants from the Sandler Family Supporting Foundation (to L. L. L.), the Ministry of Education, Science and Culture of Japan (to A. S.), and the Wellcome Trust and the National Health and Medical Research Council of Australia (to G. R. S.). DNAX Research Institute is supported by Schering-Plough Corporation (NJ).

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Authors and Affiliations

  1. Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, 305-8575, Japan

    Akira Shibuya, Norihisa Sakamoto, Yoshio Shimizu, Kazuko Shibuya, Mitsujiro Osawa, Takashi Hiroyama & Hiromitsu Nakauchi

  2. TOREST (JST), 1-1-1 Tennodai, Tsukuba Science City, Ibaraki, 305-8575, Japan

    Akira Shibuya

  3. CREST (JST), 1-1-1 Tennodai, Tsukuba Science City, Ibaraki, 305-8575, Japan

    Hiromitsu Nakauchi

  4. Department of Parasitology and Immunology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan

    Akira Shibuya, Norihisa Sakamoto, Kazuko Shibuya & Eiichi Nakayama

  5. First Department of Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan

    Norihisa Sakamoto & Takao Tsuji

  6. Department of Cytogenetics and Molecular Genetics, Centre for Medical Genetics, Women's and Children's Hospital, Adelaide, 5006, South Australia, Australia

    Helen J. Eyre & Grant R. Sutherland

  7. Department of Biochemistry II, Fukushima Medical University, Hikarigaoka, 1, Fukushima, 960-1295, Japan

    Yuichi Endo & Teizo Fujita

  8. The Second Research Department, Central Technology Laboratory, Asahi Chemical Industry Co. Ltd., 2-1 Samejima, Fuji, 416-8501, Shizuoka, Japan

    Tomoyuki Miyabayashi & Seiji Sakano

  9. Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Avenue, Palo Alto, 94304, CA, USA

    Joseph H. Phillips & Lewis L. Lanier

  10. Department of Microbiology and Immunology and the Cancer Research Institute, University of California San Francisco, 513 Parnassus Avenue, Box 0414, San Francisco, 94143-0414, CA, USA

    Lewis L. Lanier

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  1. Akira Shibuya
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Correspondence to Akira Shibuya.

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Shibuya, A., Sakamoto, N., Shimizu, Y. et al. Fcα/μ receptor mediates endocytosis of IgM-coated microbes. Nat Immunol 1, 441–446 (2000). https://doi.org/10.1038/80886

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