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RNAi screening: tips and techniques

Nature Immunology volume 10, pages 799–804 (2009)Cite this article

By identifying gene products whose knockdown is associated with phenotypic changes, large-scale RNA-mediated interference screens have demonstrated previously unknown components of biological pathways. This commentary provides general guidelines for using such screens to answer questions of immunological interest.

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Figure 1: Steps involved in RNAi screening.
Figure 2: Various types of RNAi chemistry.
Figure 3: Z-factor calculation and ranking of screen 'hits'.

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Acknowledgements

Supported by the US National Institutes of Health (A.R.), the Juvenile Diabetes Research Foundation (A.R.), the Canadian Institutes for Health Research (S.S.) and the Leukemia and Lymphoma Society (S.S.).

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Authors and Affiliations

  1. Sonia Sharma and Anjana Rao are in the Department of Pathology, Harvard Medical School, Immune Disease Institute and the Program in Cellular and Molecular Medicine, Children's Hospital Boston, Boston, Massachusetts, USA.,

    Sonia Sharma & Anjana Rao

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  1. Sonia Sharma
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Correspondence to Anjana Rao.

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Competing interests

A.R. is a shareholder of and scientific advisor to CaliMedica and receives laboratory support from Glaxo-Smith-Kline.

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Sharma, S., Rao, A. RNAi screening: tips and techniques. Nat Immunol 10, 799–804 (2009). https://doi.org/10.1038/ni0809-799

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