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RNAi screening: tips and techniques

Nature Immunology volume 10, pages 799804 (2009) | Download Citation

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By identifying gene products whose knockdown is associated with phenotypic changes, large-scale RNA-mediated interference screens have demonstrated previously unknown components of biological pathways. This commentary provides general guidelines for using such screens to answer questions of immunological interest.

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Acknowledgements

Supported by the US National Institutes of Health (A.R.), the Juvenile Diabetes Research Foundation (A.R.), the Canadian Institutes for Health Research (S.S.) and the Leukemia and Lymphoma Society (S.S.).

Author information

Affiliations

  1. Sonia Sharma and Anjana Rao are in the Department of Pathology, Harvard Medical School, Immune Disease Institute and the Program in Cellular and Molecular Medicine, Children's Hospital Boston, Boston, Massachusetts, USA.

    • Sonia Sharma
    •  & Anjana Rao

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Competing interests

A.R. is a shareholder of and scientific advisor to CaliMedica and receives laboratory support from Glaxo-Smith-Kline.

Corresponding author

Correspondence to Anjana Rao.

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DOI

https://doi.org/10.1038/ni0809-799

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