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Pursuing gene regulation 'logic' via RNA interference and chromatin immunoprecipitation

Nature Immunology volume 7, pages 692–697 (2006)Cite this article

RNA interference and chromatin immunoprecipitation are now firmly established as useful methods for studying mechanisms of gene regulation in vivo. Their combined use can help elucidate gene regulation 'logic' by aiding in target gene identification for transcription factors and chromatin-modifying complexes.

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Figure 1: Use of RNAi and ChIP to categorize LPS-induced genes in mouse macrophages on the basis of their requirement for ATP-dependent nucleosome-remodeling complexes, their kinetics of activation and their protein synthesis requirements.

Katie Ris

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Authors and Affiliations

  1. Howard Hughes Medical Institute and Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, 90095-1662, California, USA

    Caiyi C Li, Vladimir R Ramirez-Carrozzi & Stephen T Smale

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  1. Caiyi C Li
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  2. Vladimir R Ramirez-Carrozzi
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  3. Stephen T Smale
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Li, C., Ramirez-Carrozzi, V. & Smale, S. Pursuing gene regulation 'logic' via RNA interference and chromatin immunoprecipitation. Nat Immunol 7, 692–697 (2006). https://doi.org/10.1038/ni0706-692

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